ortho-Fluoroazobenzene derivatives as DNA intercalators for photocontrol of DNA and nucleosome binding by visible light

Org Biomol Chem. 2019 Feb 13;17(7):1827-1833. doi: 10.1039/c8ob02343c.

Abstract

We report a high-affinity photoswitchable DNA binder, which displays different nucleosome-binding capacities upon visible-light irradiation. Both photochemical and DNA-recognition properties were examined by UV-Vis, HPLC, CD spectroscopy, NMR, FID assays, EMSA and DLS. Our probe sets the basis for developing new optoepigenetic tools for conditional modulation of nucleosomal DNA accessibility.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Azo Compounds / chemical synthesis
  • Azo Compounds / chemistry*
  • DNA / chemistry*
  • Intercalating Agents / chemical synthesis
  • Intercalating Agents / chemistry*
  • Light*
  • Molecular Structure
  • Nucleosomes / chemistry*
  • Photochemical Processes

Substances

  • Azo Compounds
  • Intercalating Agents
  • Nucleosomes
  • DNA
  • azobenzene