Optimization of recombinant human platelet-derived growth factor-BB encapsulated in Poly (lactic-co-glycolic acid) microspheres for applications in wound healing

Yun Ju Bae; Chi Heung Cho; Woo Jong Lee; Jeung Soo Huh; Jeong Ok Lim

doi:10.1007/s13770-015-0029-z

Optimization of recombinant human platelet-derived growth factor-BB encapsulated in Poly (lactic-co-glycolic acid) microspheres for applications in wound healing

Tissue Eng Regen Med. 2016 Feb 2;13(1):13-20. doi: 10.1007/s13770-015-0029-z. eCollection 2016 Feb.

Authors

Yun Ju Bae^{1

2}, Chi Heung Cho³, Woo Jong Lee², Jeung Soo Huh⁴, Jeong Ok Lim^{1

5}

Affiliations

¹ 1Department of Biomedical Science, Graduate School, Kyungpook National University, Daegu, Korea.
² Korea Institute of Industrial Technology, Biomedical Manufacturing Technology Center, Daegu, Korea.
³ 3Department of Food Science and Biotechnology and Institute of Life Sciences and Resources, Kyung Hee University, Yongin, Korea.
⁴ 4Department of Materials Science and Metallurgy, College of Engineering, Kyungpook National University, Daegu, Korea.
⁵ 5Biomedical Research Institute, Joint Institute for Regenerative Medicine, Kyungpook National University Hospital, Daegu, Korea.

Abstract

Growth factors play multiple and critical roles in wound repair processes. Platelet-derived growth factor (PDGF) is a potent growth factor that is particularly important in the early inflammatory phase of wound healing. In order to extend the half-life of PDGF, polymeric encapsulation is used. In the current study, Poly (lactic-co-glycolic acid) (PLGA) microspheres containing recombinant human (rh) PDGF-BB were prepared to prolong the effectiveness of this growth factor. PLGA microspheres were optimized using a modified w/o/w-double-emulsion/solvent evaporation method by changing the processing conditions of stirring speed and emulsifier (polyvinyl alcohol) concentration. Microspheres prepared using the optimized method released rhPDGF-BB for up to three weeks. An in vitro migration assay showed a significant decrease in the wound area in cells treated with rhPDGF-BB microspheres compared to control cells. These findings demonstrate the potential of rhPDGF-BB encapsulated in microspheres to enhance wound healing.

Keywords: Human immortalized keratinocyte cell line; Migration; Poly (lactic-co-glycolic acid) microsphere; Recombinant human platelet-derived growth factor-BB; Wound healing.