Promotion of excisional wound repair by a menstrual blood-derived stem cell-seeded decellularized human amniotic membrane

Saeed Farzamfar; Majid Salehi; Arian Ehterami; Mahdi Naseri-Nosar; Ahmad Vaez; Amir Hassan Zarnani; Hamed Sahrapeyma; Mohammad-Reza Shokri; Mehdi Aleahmad

doi:10.1007/s13534-018-0084-1

Promotion of excisional wound repair by a menstrual blood-derived stem cell-seeded decellularized human amniotic membrane

Biomed Eng Lett. 2018 Sep 11;8(4):393-398. doi: 10.1007/s13534-018-0084-1. eCollection 2018 Nov.

Authors

Saeed Farzamfar^#¹, Majid Salehi^#^{2

3}, Arian Ehterami^#², Mahdi Naseri-Nosar^#³, Ahmad Vaez¹, Amir Hassan Zarnani^{4

5}, Hamed Sahrapeyma⁶, Mohammad-Reza Shokri⁷, Mehdi Aleahmad⁴

Affiliations

¹ 1Department of Tissue Engineering and Applied Cell Sciences, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
² 2Department of Tissue Engineering, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran.
³ 3Tissue Engineering and Stem Cells Research Center, Shahroud University of Medical Sciences, Shahroud, Iran.
⁴ 4Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
⁵ 5Reproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran.
⁶ 6Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran.
⁷ 7Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.

^# Contributed equally.

Abstract

This is the first study demonstrating the efficacy of menstrual blood-derived stem cell (MenSC) transplantation via decellularized human amniotic membrane (DAM), for the promotion of skin excisional wound repair. The DAM was seeded with MenSCs at the density of 3 × 10⁴ cells/cm² and implanted onto a rat's 1.50 × 1.50 cm² full-thickness excisional wound defect. The results of wound closure and histopathological examinations demonstrated that the MenSC-seeded DAM could significantly improve the wound healing compared with DAM-treatment. All in all, our data indicated that the MenSCs can be a potential source for cell-based therapies to regenerate skin injuries.

Keywords: Amniotic membrane; Menstrual blood-derived stem cells; Wound healing.