Background: Fractalkine (FKN) is an inflammatory cytokine that has been shown with increased serum levels in diabetic patients and is considered to contribute to the adipose tissue inflammation by supporting monocyte adhesion to adipocytes which has an important role in the pathogenesis of type 2 diabetes mellitus (T2DM). Our aim was to evaluate the effects of glucose ingestion on the serum fractal - kine levels in healthy subjects with normal glucose tolerance (NGT) and newly diagnosed T2DM patients.
Methods: A total of 67 patients were included in this study, and they were divided into NGT (n=34) and T2DM (n=33) groups according to their oral glucose tolerance test (OGTT) results. The serum FKN and C-reactive protein (CRP) levels were measured at 0 and 120 minutes during an OGTT following overnight fasting.
Results: The 0-minute (basal) and 120-minute OGTT FKN levels were found to be significantly higher in the T2DM group when compared to the NGT group (p=0.012 and p=0.001, respectively). However, no significant differences were observed in terms of the changes in the basal and 120-minute OGTT FKN levels in the T2DM and NGT groups (p=0.433 and p=0.06, respectively). A significant positive correlation was observed between the 120-minute OGTT FKN and glucose levels in the study group consisting of all of the patients (r=0.331, p=0.006).
Conclusions: In this study, basal and post-glycemic load FKN levels were found to be higher in newly diagnosed T2DM patients than those with NGT; however, there was no additional change in FKN levels by glycemic load.
Uvod: Fraktalkin (FKN) jeste inflamatorni citokin otkriven u povišenim serumskim nivoima kod obolelih od dijabetesa i za koji se smatra da doprinosi inflamaciji adipoznog tkiva tako što podstiče adheziju monocita za adipocite što ima važnu ulogu u patogenezi dijabetes melitusa tipa 2 (T2DM). Naš cilj bio je procena efekata unosa glukoze na nivo serumskog FKN-a kod zdravih ispitanika sa normalnom tolerancijom glukoze (NGT) i pacijenata sa T2DM.
Metode: Ukupno 67 pacijenata je obuhvaćeno u ovoj studiji i podeljeni su na NGT (n = 34) i T2DM (n = 33) grupe u skladu sa rezultatima oralnog testa tolerancije glukoze (OGTT). Nivoi serumskog FKN-a i C-reaktivnog proteina (CRP) mereni su na 0 i 120 minuta tokom OGTT-a nakon noćnog posta.
Rezultati: Utvrđeno je da su 0-minutni (bazalni) i 120-minutni nivoi OGTT FKN-a znatno viši u T2DM grupi u odnosu na NGT grupu (p = 0,012 i p = 0,001). Međutim, nisu zabeležene značajne razlike u pogledu promena u bazalnom i 120-minutnom nivou OGTT FKN-a u T2DM i NGT grupama (p = 0,433 i p = 0,06). Značajna pozitivna korelacija je utvrđena između 120-minutnog OGTT FKN-a i nivoa glukoze u studijskoj grupi koja se sastoji od svih pacijenata (r = 0,331, p = 0,006).
Zaključak: Prema našem saznanju, efekti unosa glukoze na nivo serumskog FKN-a su prvi put procenjeni u ovoj studiji. U ovoj studiji, bazalni nivoi FKN-a i nivoi posle opterećenja glukozom bili su viši kod pacijenata sa novodijagnostikovanim T2DM nego sa NGT, ali nije bilo dodatnih promena nivoa FKN-a u odnosu na opterećenje glukozom.
Keywords: fractalkine; inflammation; oral glucose tolerance test; type 2 diabetes mellitus.