Chemical composition, antioxidant and antitumor activities of sub-fractions of wild and cultivated Pleurotus ferulae ethanol extracts

Yi Yang; Changshuang Fu; Fangfang Zhou; Xiaoyu Luo; Jinyu Li; Jun Zhao; Jiang He; Xiaoqin Li; Jinyao Li

doi:10.7717/peerj.6097

Chemical composition, antioxidant and antitumor activities of sub-fractions of wild and cultivated Pleurotus ferulae ethanol extracts

PeerJ. 2018 Dec 20:6:e6097. doi: 10.7717/peerj.6097. eCollection 2018.

Authors

Yi Yang¹, Changshuang Fu¹, Fangfang Zhou¹, Xiaoyu Luo¹, Jinyu Li², Jun Zhao³, Jiang He³, Xiaoqin Li⁴, Jinyao Li^{1

4}

Affiliations

¹ College of Life Science and Technology, Xinjiang University, Urumqi, China.
² College of Life Science, Xinjiang Normal University, Urumqi, China.
³ Key Laboratory for Uighur Medicine, Institute of Materia Medica of Xinjiang, Urumqi, China.
⁴ Affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, China.

Abstract

Pleurotus ferulae is an edible and medicinal mushroom with various bioactivities. Here, the ethanol extracts of wild and cultivated P. ferulae (PFEE-W and PFEE-C) and their subfractions including petroleum ether (Pe-W/Pe-C), ethyl acetate (Ea-W/Ea-C) and n-butanol (Ba-W/Ba-C) were prepared to evaluate their antioxidant and antitumor activities. Both PFEE-W and PFEE-C show the antioxidant activity and PFEE-W is stronger than PFEE-C. The antioxidant activities of their subfractions are in the following order: Ea > Ba > Pe. Moreover, PFEE-W and PFEE-C significantly inhibit the proliferation of murine melanoma B16 cells, human esophageal cancer Eca-109 cells, human gastric cancer BGC823 cells and human cervical cancer HeLa cells through induction of apoptosis, which partially mediated by reactive oxygen species. The antitumor activities of their subfractions are in the following order: Ea ≥ Pe > Ba. Pe-W shows higher antitumor activity compared with Pe-C, which might be correlated with the difference of their components identified by gas chromatography-mass spectrometry. These results suggest that both wild and cultivated P. ferulae have antioxidant and antitumor activities, and cultivated P. ferulae could be used to replace wild one in some functions.

Keywords: Antioxidation; Antitumor; Apoptosis; Ethanol extract; Pleurotus ferulae.

Grants and funding

This work was supported by the “13th Five-Year” Plan for Key Discipline Biology Bidding Project (17SDKD0202), Xinjiang Normal University to Jinyu Li and the Chinese National Natural Science Foundation Grant (31460241) and the High Level Talent Introduction Project of Xinjiang Uygur Autonomous Region to Jinyao Li. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.