Poly-adenine-mediated spherical nucleic acids for strand displacement-based DNA/RNA detection

Lu Wang; Huan Zhang; Chenguang Wang; Yi Xu; Jing Su; Xiao Wang; Xinxin Liu; Dezhi Feng; Lihua Wang; Xiaolei Zuo; Jiye Shi; Zhilei Ge; Chunhai Fan; Xianqiang Mi

doi:10.1016/j.bios.2018.12.003

Poly-adenine-mediated spherical nucleic acids for strand displacement-based DNA/RNA detection

Biosens Bioelectron. 2019 Feb 15:127:85-91. doi: 10.1016/j.bios.2018.12.003. Epub 2018 Dec 7.

Authors

Lu Wang¹, Huan Zhang², Chenguang Wang², Yi Xu², Jing Su², Xiao Wang², Xinxin Liu², Dezhi Feng², Lihua Wang³, Xiaolei Zuo⁴, Jiye Shi³, Zhilei Ge⁵, Chunhai Fan⁶, Xianqiang Mi⁷

Affiliations

¹ Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201220, China; University of Chinese Academy of Sciences, Beijing 100049, China.
² Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201220, China.
³ Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
⁴ School of Chemistry and Chemical Engineering, Institute of Molecular Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200240, China.
⁵ Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China; School of Chemistry and Chemical Engineering, Institute of Molecular Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: gezhilei@sinap.ac.cn.
⁶ Division of Physical Biology & Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China; School of Chemistry and Chemical Engineering, Institute of Molecular Medicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200240, China.
⁷ Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201220, China. Electronic address: mixq@sari.ac.cn.

PMID: 30594078
DOI: 10.1016/j.bios.2018.12.003

Abstract

DNA-gold nanoparticles (AuNPs) conjugate is one of the most versatile bionanomaterials for biomedical and clinical diagnosis. However, to finely tune the hybridization ability and precisely control the orientation and conformation of surface-tethered oligonucleotides on AuNPs remains a hurdle. In this work, we developed a poly adenine-mediated spherical nucleic acid (polyA-mediated SNA) strategy by assembling di-block DNA probes on gold nanoparticles (AuNPs) to spatially control interdistance and hybridization ability of oligonucleotides on AuNPs. By modulating length of poly A bound on the SNA with different degrees of constructing, we presented significant improved biosensing performance including high hybridization efficiency, and expanded dynamic range of analytes with more sensitive detection limit. Furthermore, this polyA design could facilitate the programmable detection for DNA in serum environment and simultaneous multicolor detection of three different microRNAs associated with pancreatic carcinoma. The demonstration of the link between modulation of SNA assembly strategy and biodetection capability will increase the development of high performance diagnostic tools for translational biomedicine.

Keywords: Bioanalytical chemistry; DNA strand displacement; DNA/microRNA detection; Multiplexing detection; PolyA-AuNPs.

MeSH terms

Biosensing Techniques*
DNA / chemistry
DNA / isolation & purification*
Humans
Nucleic Acid Hybridization
Oligonucleotides / chemistry
Poly A / chemistry*
RNA / chemistry
RNA / isolation & purification*

Substances

Oligonucleotides
polyadenine
Poly A
RNA
DNA