Inspecting human colon adenocarcinoma cell lines by using terahertz time-domain reflection spectroscopy

Yuqi Cao; Jiani Chen; Pingjie Huang; Weiting Ge; Dibo Hou; Guangxin Zhang

doi:10.1016/j.saa.2018.12.023

Inspecting human colon adenocarcinoma cell lines by using terahertz time-domain reflection spectroscopy

Spectrochim Acta A Mol Biomol Spectrosc. 2019 Mar 15:211:356-362. doi: 10.1016/j.saa.2018.12.023. Epub 2018 Dec 11.

Authors

Yuqi Cao¹, Jiani Chen², Pingjie Huang³, Weiting Ge², Dibo Hou¹, Guangxin Zhang⁴

Affiliations

¹ State Key Laboratory of Industrial Control Technology, College of Control Science and Engineering, Zhejiang University, Hangzhou, People's Republic of China.
² Cancer Institute, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, People's Republic of China.
³ State Key Laboratory of Industrial Control Technology, College of Control Science and Engineering, Zhejiang University, Hangzhou, People's Republic of China. Electronic address: huangpingjie@zju.edu.cn.
⁴ State Key Laboratory of Industrial Control Technology, College of Control Science and Engineering, Zhejiang University, Hangzhou, People's Republic of China. Electronic address: gxzhang@zju.edu.cn.

PMID: 30593945
DOI: 10.1016/j.saa.2018.12.023

Abstract

Techniques to inspect and analyze human colorectal cancer cell lines by using terahertz time-domain attenuated total reflection spectroscopy (THz TD-ATR) were investigated. The characteristics of THz absorption spectra of two colorectal cancer cell lines DLD-1 and HT-29 in aqueous solutions with different concentrations were studied. Different spectral features were observed compared to normal cell line. Identification results based on different parameters including absorption coefficient, refractive index, real and imaginary parts of complex permittivity, dielectric loss tangent were discussed. This research may be promising for quick and instant inspection of liquid samples by using THz time-domain spectroscopy in medical applications.

Keywords: Absorption coefficient; Cancer identification; DLD-1 cell line; Dielectric loss tangent; HT-29 cell line; Terahertz time-domain attenuated total reflection spectroscopy.

MeSH terms

Adenocarcinoma / chemistry*
Adenocarcinoma / diagnosis
Adenocarcinoma / pathology
Animals
Cell Line, Tumor
Colonic Neoplasms / chemistry*
Colonic Neoplasms / diagnosis
Colonic Neoplasms / pathology
HT29 Cells
Humans
Mice
NIH 3T3 Cells
Terahertz Spectroscopy / methods*