Lysophosphatidic acid precursor levels decrease and an arachidonic acid-containing phosphatidylcholine level increases in the dorsal root ganglion of mice after peripheral nerve injury

Yuki Mihara; Makoto Horikawa; Shumpei Sato; Fumihiro Eto; Mitsuru Hanada; Tomohiro Banno; Hideyuki Arima; Hiroki Ushirozako; Tomohiro Yamada; Dongmin Xu; Ayako Okamoto; Fumiyoshi Yamazaki; Shiro Takei; Takao Omura; Ikuko Yao; Yukihiro Matsuyama; Mitsutoshi Setou

doi:10.1016/j.neulet.2018.12.035

Lysophosphatidic acid precursor levels decrease and an arachidonic acid-containing phosphatidylcholine level increases in the dorsal root ganglion of mice after peripheral nerve injury

Neurosci Lett. 2019 Apr 17:698:69-75. doi: 10.1016/j.neulet.2018.12.035. Epub 2018 Dec 26.

Authors

Yuki Mihara¹, Makoto Horikawa², Shumpei Sato², Fumihiro Eto³, Mitsuru Hanada¹, Tomohiro Banno¹, Hideyuki Arima¹, Hiroki Ushirozako¹, Tomohiro Yamada¹, Dongmin Xu¹, Ayako Okamoto¹, Fumiyoshi Yamazaki², Shiro Takei⁴, Takao Omura¹, Ikuko Yao⁵, Yukihiro Matsuyama¹, Mitsutoshi Setou⁶

Affiliations

¹ Department of Orthopedic Surgery, Hamamatsu University School of Medicine, 1-20-1, Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan.
² International Mass Imaging Center and Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan.
³ International Mass Imaging Center and Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan; Department of Optical Imaging, Institute for Medical Photonics Research, Preeminent Medical Photonics Education & Research Center, Japan.
⁴ International Mass Imaging Center and Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan; Department of Environmental Biology, College of Bioscience and Biotechnology, Chubu University, 1200 Matsumoto-cho, Kasugai, Aichi, 487-8501, Japan.
⁵ Department of Optical Imaging, Institute for Medical Photonics Research, Preeminent Medical Photonics Education & Research Center, Japan.
⁶ International Mass Imaging Center and Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan; Preeminent Medical Photonics Education & Research Center, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka, 431-3192, Japan; Department of Anatomy, The University of Hong Kong, 6/F, William MW Mong Block 21 Sassoon Road, Pokfulam, Hong Kong SAR, China. Electronic address: setou@hama-med.ac.jp.

PMID: 30593874
DOI: 10.1016/j.neulet.2018.12.035

Abstract

In the current study, we aimed to analyze the lipid changes in the dorsal root ganglion (DRG) after sciatic nerve transection (SNT) using matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI-IMS). We found that the arachidonic acid-containing phosphatidylcholine (AA-PC), PC(16:0/20:4) largely increased, while PC(16:0/18:1), PC(18:0/18:1) and phosphatidic acid (PA)(36:2) levels largely decreased in the DRG following nerve injury. Previous studies show that the increase in PC(16:0/20:4) was associated with neuropathic pain and that decrease in PC(16:0/18:1), PC(18:0/18:1), and PA(36:2) were due to producing lysophosphatidic acid (LPA), an initiator for neuropathic pain. These results suggest that the lipid changes in DRG after SNT could be the result of changes for the cause of neuropathic pain. Thus, blocking of LPA could be potential for treatment of neuropathic pain.

Keywords: Imaging mass spectrometry; Lysophosphatidic acid; Matrix-assisted laser desorption ionization; Neuropathic pain; Peripheral nerve injury; Sciatic nerve transection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Arachidonic Acid / metabolism*
Ganglia, Spinal / metabolism*
Lysophospholipids / metabolism*
Mice, Inbred C57BL
Neuralgia / metabolism
Phosphatidic Acids / metabolism
Phosphatidylcholines / metabolism*
Sciatic Nerve / injuries
Sciatic Nerve / metabolism

Substances

Lysophospholipids
Phosphatidic Acids
Phosphatidylcholines
Arachidonic Acid
lysophosphatidic acid