Investigating Hepatitis C Virus Infection Using Super-Resolution Microscopy

Pedro Matos Pereira; Caron Jacobs; Joe Grove

doi:10.1007/978-1-4939-8976-8_17

Investigating Hepatitis C Virus Infection Using Super-Resolution Microscopy

Methods Mol Biol. 2019:1911:247-261. doi: 10.1007/978-1-4939-8976-8_17.

Authors

Pedro Matos Pereira¹, Caron Jacobs¹, Joe Grove²

Affiliations

¹ Quantitative Imaging and Nanobiophysics Group, MRC Laboratory for Molecular Cell Biology, Department of Cell and Developmental Biology, University College London, London, UK.
² Division of Infection and Immunity, Institute of Immunity and Transplantation, University College London, London, UK. j.grove@ucl.ac.uk.

PMID: 30593631
DOI: 10.1007/978-1-4939-8976-8_17

Abstract

Super-resolution microscopy (SRM) can provide a window on the nanoscale events of virus replication. Here we describe a protocol for imaging hepatitis C virus-infected cells using localization SRM. We provide details on sample preparation, immunostaining, data collection, and super-resolution image reconstruction. We have made all efforts to generalize the protocol to make it accessible to all budding super-resolution microscopists.

Keywords: Hepatitis; Imaging; Microscopy; Super-resolution; Virus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Hepacivirus / isolation & purification*
Hepatitis C / pathology*
Humans
Image Processing, Computer-Assisted / methods*
Microscopy, Fluorescence / methods*
Staining and Labeling / methods

Abstract

Publication types

MeSH terms

Grants and funding