Isolation, genetic identification and degradation characteristics of COD-degrading bacterial strain in slaughter wastewater

Saudi J Biol Sci. 2018 Dec;25(8):1800-1805. doi: 10.1016/j.sjbs.2018.08.022. Epub 2018 Aug 23.

Abstract

Contamination of water by meat production is an important and extensive environmental problem and even threat to human health. Biodegradation is a major mechanism which removes the pollutants from the environment. Therefore, the present study aimed to isolate and characterize a COD degrading bacteria which can effectively degrade slaughter wastewater. Six COD degrading bacteria were isolated from slaughtering waste water and sludge in Hunan a meat product Co., Ltd. And the COD degradation rate of each strain was determined by potassium permanganate method. Through observing morphologically and analyzing sequence to 16S rDNA, the highest COD degradation strain was Bacillus velezensis by preliminarily identified and classified, reaching 11.80%. The suitable conditions of the growth of Bacillus velezensis strain were 37 °C, pH 7.0, the peptone concentration 1.5%, and the yeast extract concentration 0.8%.

Keywords: Bacillus velezensis; Characterization; Degrading bacteria; Identification; Slaughter wastewater.