Altered Expression of PYCARD, Interleukin 1β, Interleukin 18, and NAIP in Successfully Treated HIV-Positive Patients With a Low Ratio of CD4+ to CD8+ T Cells

J Infect Dis. 2019 May 5;219(11):1743-1748. doi: 10.1093/infdis/jiy730.

Abstract

The expression and activity of main inflammasome components in monocytes from successfully treated human immunodeficiency virus (HIV)-positive patients are poorly studied. Thus, we enrolled 18 patients with a low and 17 with a normal ratio of CD4+ T cells to CD8+ T cells and 11 healthy donors. We found that patients with a low ratio had decreased CCR2 expression among classical and intermediate monocytes and increased CCR5 expression among classical monocytes, compared with patients with a normal ratio. Patients with a low ratio also had higher NAIP and PYCARD messenger RNA levels after lipopolysaccharide stimulation, suggesting an altered ability to control immune activation that could affect their immune reconstitution.

Keywords: AIM2; CD4/CD8 ratio; HIV; IL-18; IL-1β; Inflammasome; NAIP; PYCARD; monocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • CARD Signaling Adaptor Proteins / genetics*
  • CD4-Positive T-Lymphocytes / immunology
  • CD8-Positive T-Lymphocytes / immunology
  • Female
  • Gene Expression Regulation
  • HIV Infections / immunology*
  • HIV Infections / virology
  • HIV Seropositivity
  • Humans
  • Inflammasomes / immunology*
  • Interleukin-18 / genetics*
  • Interleukin-1beta / genetics*
  • Male
  • Middle Aged
  • Monocytes / immunology
  • Neuronal Apoptosis-Inhibitory Protein / genetics*

Substances

  • CARD Signaling Adaptor Proteins
  • IL18 protein, human
  • IL1B protein, human
  • Inflammasomes
  • Interleukin-18
  • Interleukin-1beta
  • NAIP protein, human
  • Neuronal Apoptosis-Inhibitory Protein
  • PYCARD protein, human