Biomolecule Sensor Based on Azlactone-Modified Hydrogel Films

Macromol Rapid Commun. 2019 Apr;40(7):e1800674. doi: 10.1002/marc.201800674. Epub 2018 Dec 27.

Abstract

A 3D hydrogel layer is probed by combining surface plasmon resonance with optical waveguide spectroscopy to detect biomolecules. A template terpolymer P(DMAAm-co-DMIAAm-co-VDMA) is synthesized via reversible addition-fragmentation chain-transfer polymerization. The terpolymer is then modified with an amino group bearing biotin to enable biomolecular recognition for streptavidin. A hydrogel thin layer is prepared onto a gold surface after spin-coating and photo-crosslinking of the modified polymer. Finally, the hydrogel is utilized to quantitatively detect streptavidin by using surface plasmon resonance-optical waveguide spectroscopy measurements.

Keywords: azlactone; biotin and streptavidin; hydrogels; sensors; surface plasmon resonance.

MeSH terms

  • Gold / chemistry
  • Hydrogel, Polyethylene Glycol Dimethacrylate / chemical synthesis
  • Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry*
  • Lactones / chemistry*
  • Molecular Structure
  • Polymerization
  • Polymers / chemical synthesis*
  • Polymers / chemistry
  • Surface Plasmon Resonance
  • Surface Properties

Substances

  • Lactones
  • Polymers
  • Hydrogel, Polyethylene Glycol Dimethacrylate
  • Gold