Structural analysis of 25-hydroxycholesterol stereoisomers differing in configuration in position 17 and 20, by three-dimensional NMR spectra

Steroids. 2019 Mar:143:49-52. doi: 10.1016/j.steroids.2018.12.005. Epub 2018 Dec 22.

Abstract

The application of 3D NMR experiments and DFT calculations enabled the structure investigation of C-17 epimer of 3-(25-hydroxycholest-5-enyl) acetate is presented. The H-17 and H-20 protons features the same values of 1H chemical shift, what causes that the structure elucidation require additional resolution enabled by 3D NMR experiments. The NMR experiments and theoretical calculations allowed for: the resonance assignment (3D COSY-HMBC and 3D TOCSY-HSQC techniques), the prediction of spatial structure (3D NOESY-HSQC and 3D ROESY-HSQC experiments), and the precise measurement of heteronuclear coupling constants (3D HSQC-TOCSY spectra with E.COSY-type multiplets).

Keywords: 3D NMR; DFT calculations; Non-uniform sampling; Steroids; Structure determination.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Hydroxycholesterols / chemistry*
  • Magnetic Resonance Spectroscopy
  • Models, Molecular
  • Molecular Conformation
  • Stereoisomerism

Substances

  • Hydroxycholesterols
  • 25-hydroxycholesterol