Background: The exact role of cytokines in inflammation and metabolic disorders in case of connective tissue diseases (CTDs) is under discussion.
Methods: In this study, we intended to find the relationship between the selected cytokines in inflammatory and metabolic disorders in patients with CTDs (n=55) and compared the results with those of control group subjects (n=25) matched by age and body mass. We estimated their nutritional status by the bioimpedance method. The levels of basic biochemical parameters and the levels of adiponectin, resistin, and chemerin were also estimated. Multiple regressions and area under the curve in receiver operating characteristic (AUC-ROC) curve were used to find the associations of aforementioned parameters.
Results: Patients with CTDs exhibited higher levels of chemerin than that of control group subjects. We found an inverse relationship between chemerin, RBC count, and hemoglobin levels. The concentration of adiponectin inversely correlated with the levels of platelets and concentrations of glucose and triglycerides as well as the erythrocyte sedimentation rate, whereas the concentration of resistin was positively correlated with WBC count, C-reactive protein (CRP), and the amount of used oral glucocorticosteroids. The mean ± standard deviation for the AUC-ROC curve in case of chemerin was the highest (AUC-ROC=0.714, p=0.0005) than that of both resistin and adiponectin.
Conclusions: Chemerin and resistin levels are related to the inflammatory state in patients with CTDs, whereas adiponectin levels seem to be correlated with a protective effect. Chemerin can be considered as a marker differentiating a proinflammatory state present in CTDs.
Uvod: Tačna uloga citokina kod inflamatornih i metaboličkih poremećaja kod oboljenja konektivnih tkiva (CTD) je diskutabilna.
Metode: U ovom istraživanju, ispitivan je odnos između pojedinih citokina kod pacijenata sa inflamatornim i metaboličkim poremećajima sa CTD (n= 55) i poređen sa rezultatima istih kod kontrolne grupe (n= 25) shodno godinama starosti i telesnoj težini. Ispitivan je njihov nutricioni status metodom bioimpendance. Određivani su nivoi osnovnih biohemijskih parametara, adiponektina, rezistina i chemerina. Za obradu podataka korišćena je multipla regresiona analiza sa površinom ispod krive (AUC-ROC).
Rezultati: Pacijenti sa CTD imali su više nivoe chemerina nego osobe u kontrolnoj grupi. Utvrđen je obrnut odnos između chemerina, broja eritrocita i nivoa hemoglobina. Koncentracija adiponektina bila je u obrnutoj korelaciji sa nivoima trombocita i koncentracijama glukoze i triglicerida kao i sa sedimentacijom eritrocita, dok je koncentracija rezistina bila u pozitivnoj korelaciji sa brojem leukocita, C-reaktivnim proteinom (CRP) i količinom oralno korišćenih glukokortikosteroida. Srednja vrednost ± standardna devijacija za AUC-ROC krivu u slučaju chemerina bila je veća (AUC-ROC = 0,714, p = 0,0005) nego kod rezistina i adiponektina.
Zaključak: Nivoi chemerina i rezistina bili su u korelaciji sa inflamatornim stanjem pacijenata sa CTD, dok su nivoi adiponektina bili u korelaciji sa zaštitnim efektom. Može da se smatra da je chemerin marker proinflamtornog stanja u CTD.
Keywords: connective tissue diseases; cytokines; inflammation; nutritional status.