Abstract
A new sesquiterpene (1) and a new monoterpene (2), together with thirteen known compounds (3-15) were isolated from an ethanol extract of the roots of Aristolochia debilis Sieb. et Zucc. The structures of compounds 1 and 2 were elucidated using HR-ESI-MS, 1D and 2D NMR spectroscopy. Anti-inflammatory effects of the isolated compounds were evaluated in terms of inhibition of nitric oxide, tumour-necrosis factor-α and interleukin-6 production in lipopolysaccharide-stimulated RAW264.7 cells. Compounds 1-9 and 12-15 significantly inhibited the levels of NO, TNF-α and IL-6 in LPS-induced RAW264.7 cells from concentrations of 3 μM to 30 μM.
Keywords:
Aristolochia debilis; Aristolochiaceae; anti-inflammatory; monoterpene; sesquiterpene.
MeSH terms
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Animals
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Anti-Inflammatory Agents / chemistry
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Anti-Inflammatory Agents / isolation & purification*
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Anti-Inflammatory Agents / pharmacology
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Aristolochia / chemistry*
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Interleukin-6 / antagonists & inhibitors
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Interleukin-6 / biosynthesis
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Lipopolysaccharides
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Mice
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Molecular Structure
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Monoterpenes / chemistry
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Monoterpenes / isolation & purification*
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Monoterpenes / pharmacology
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Nitric Oxide / antagonists & inhibitors
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Nitric Oxide / biosynthesis
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Plant Roots / chemistry*
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RAW 264.7 Cells
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Sesquiterpenes / chemistry
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Sesquiterpenes / isolation & purification*
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Sesquiterpenes / pharmacology
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Spectrum Analysis / methods
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Tumor Necrosis Factor-alpha / antagonists & inhibitors
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Tumor Necrosis Factor-alpha / biosynthesis
Substances
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Anti-Inflammatory Agents
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Interleukin-6
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Lipopolysaccharides
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Monoterpenes
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Sesquiterpenes
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Tumor Necrosis Factor-alpha
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interleukin-6, mouse
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Nitric Oxide