Quantification of Legionella DNA certified reference material by digital droplet PCR

Maud Baume; Astrid Cariou; Adélaïde Leveau; Noémie Fessy; Frédéric Pastori; Sophie Jarraud; Sophie Pierre

doi:10.1016/j.mimet.2018.12.019

Quantification of Legionella DNA certified reference material by digital droplet PCR

J Microbiol Methods. 2019 Feb:157:50-53. doi: 10.1016/j.mimet.2018.12.019. Epub 2018 Dec 19.

Authors

Maud Baume¹, Astrid Cariou², Adélaïde Leveau², Noémie Fessy³, Frédéric Pastori², Sophie Jarraud³, Sophie Pierre²

Affiliations

¹ Hospices Civils de Lyon, Centre National de Référence des Légionelles, Lyon, France. Electronic address: maud.baume@chu-lyon.fr.
² Bio-Rad, Food Science Division, Marnes-la-Coquette, France.
³ Hospices Civils de Lyon, Centre National de Référence des Légionelles, Lyon, France.

PMID: 30578889
DOI: 10.1016/j.mimet.2018.12.019

Abstract

A value was assigned in 2009 to the Legionella DNA Certified Reference Material, and the stability study conducted using quantitative PCR found a low level of degradation. Herein, the Digital Droplet PCR method for Legionella DNA was qualified and used to provide absolute quantification of the CRM.

Keywords: Digital droplet PCR; Environment; Legionella; Quantification; Reference material.

MeSH terms

DNA Fragmentation*
DNA, Bacterial / genetics
DNA, Bacterial / standards*
Legionella / genetics*
Real-Time Polymerase Chain Reaction
Reference Standards

Substances

DNA, Bacterial