Combinatorial Synthesis of Peptoid Arrays via Laser-Based Stacking of Multiple Polymer Nanolayers

Daniela S Mattes; Bettina Streit; Dhaka R Bhandari; Juliane Greifenstein; Tobias C Foertsch; Stephan W Münch; Barbara Ridder; Clemens V Bojničić-Kninski; Alexander Nesterov-Mueller; Bernhard Spengler; Ute Schepers; Stefan Bräse; Felix F Loeffler; Frank Breitling

doi:10.1002/marc.201800533

Combinatorial Synthesis of Peptoid Arrays via Laser-Based Stacking of Multiple Polymer Nanolayers

Macromol Rapid Commun. 2019 Mar;40(6):e1800533. doi: 10.1002/marc.201800533. Epub 2018 Dec 21.

Authors

Daniela S Mattes^{1

2}, Bettina Streit^{1

3}, Dhaka R Bhandari⁴, Juliane Greifenstein¹, Tobias C Foertsch¹, Stephan W Münch², Barbara Ridder², Clemens V Bojničić-Kninski¹, Alexander Nesterov-Mueller¹, Bernhard Spengler⁴, Ute Schepers³, Stefan Bräse^{2

3}, Felix F Loeffler⁵, Frank Breitling¹

Affiliations

¹ Institute of Microstructure Technology, Karlsruhe Institute of Technology, Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
² Institute of Organic Chemistry, Karlsruhe Institute of Technology, Fritz-Haber-Weg 6, 76131, Karlsruhe, Germany.
³ Institute of Toxicology and Genetics, Karlsruhe Institute of Technology, Hermann-von Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
⁴ Institute of Inorganic and Analytical Chemistry, Justus-Liebig University Giessen, Heinrich-Buff-Ring 17, 35392, Giessen, Germany.
⁵ Max Planck Institute of Colloids and Interfaces, Am Mühlenberg 1, 14476, Potsdam, Germany.

PMID: 30576035
DOI: 10.1002/marc.201800533

Abstract

Here, the combinatorial synthesis of molecule arrays via a laser-assisted process is reported. Laser-transferred polymer nanolayers with embedded monomers, activators, or bases can be reliably stacked on top of each other, spot-by-spot, to synthesize molecule arrays. These various chemicals in the nanometer-thin layers are mixed by heat or solvent vapor, inducing coupling reactions. As an example, peptoid arrays with a density of 10 000 spots per cm² with the sub-monomer or monomer method are generated. Moreover, successful reactions spot-by-spot are verified by laser-transferring MALDI-matrix (Matrix-assisted laser desorption/ionization) followed by MALDI mass spectrometry imaging.

Keywords: MALDI-MS imaging; high-throughput screening; laser-induced forward transfer; molecule arrays; submonomer method.

MeSH terms

Lasers*
Molecular Structure
Nanostructures / chemistry*
Peptoids / chemical synthesis*
Peptoids / chemistry
Polymers / chemical synthesis*
Polymers / chemistry
Protein Array Analysis*

Substances

Peptoids
Polymers

Abstract

MeSH terms

Substances

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