A Gram-stain-negative, aerobic, light-pink, short rod-shaped, non-spore-forming bacterial strain was isolated from biological soil crust sampled in the Hopq Desert, Inner Mongolia, China, designated MIMBbqt21T. The G+C content of the genomic DNA was 55.1 mol%. Phylogenetic analysis of the 16S rRNA gene sequence showed that strain MIMBbqt21T belonged to the genus Hymenobacter and had the highest sequence similarity to Hymenobacter cavernaeK1E01-27T (94.35 %). Cell growth could be observed at 4-29 °C (optimum, 24 °C), pH of 6.0-8.6 (optimum, 6.0) and in the presence of 1 % (w/v) NaCl (optimum, 0 %). The major fatty acids of strain MIMBbqt21T were iso-C15 : 0, C16 : 1ω5c and summed feature 3 (C16 : 1ω7c/C16 : 1ω6c). The main polar lipids were phosphatidylethanolamine, five unidentified aminophospholipids, an unidentified glycolipid and four unidentified polar lipids. The sole respiratory quinone was menaquinone MK-7. Based on the results of the phylogenetic, chemotaxonomic and phenotypic studies, strain MIMBbqt21T could be distinguished from all known Hymenobacter species and represents a novel species, for which the name Hymenobactercrusticola sp. nov. is proposed. The type strain is MIMBbqt21T (=MCCC 1K01312T=KCTC 42804T).
Keywords: Hymenobacter crusticola; Hymenobacteraceae; biological soil crusts.