Polyelectrolyte-coated ultra-small nanoparticles with Tb(III)-centered luminescence as cell labels with unusual charge effect on their cell internalization

Rustem R Zairov; Anastasiya O Solovieva; Nataliya A Shamsutdinova; Sergey N Podyachev; Michael A Shestopalov; Tatiana N Pozmogova; Svetlana M Miroshnichenko; Asiya R Mustafina; Andrey A Karasik

doi:10.1016/j.msec.2018.10.084

Polyelectrolyte-coated ultra-small nanoparticles with Tb(III)-centered luminescence as cell labels with unusual charge effect on their cell internalization

Mater Sci Eng C Mater Biol Appl. 2019 Feb 1:95:166-173. doi: 10.1016/j.msec.2018.10.084. Epub 2018 Oct 26.

Authors

Rustem R Zairov¹, Anastasiya O Solovieva², Nataliya A Shamsutdinova³, Sergey N Podyachev³, Michael A Shestopalov⁴, Tatiana N Pozmogova⁵, Svetlana M Miroshnichenko⁶, Asiya R Mustafina³, Andrey A Karasik³

Affiliations

¹ A. E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center of Russian Academy of Sciences, Arbuzov str., 8, 420088 Kazan, Russian Federation; Kazan (Volga region) Federal University, Kremlyovskaya str., 18, 420008 Kazan, Russian Federation. Electronic address: rustem@iopc.ru.
² Scientific Institute of Clinical and Experimental Lymphology - Branch of the ICG SB RAS, 2 Timakova str., 630060 Novosibirsk, Russian Federation.
³ A. E. Arbuzov Institute of Organic and Physical Chemistry, Kazan Scientific Center of Russian Academy of Sciences, Arbuzov str., 8, 420088 Kazan, Russian Federation.
⁴ Scientific Institute of Clinical and Experimental Lymphology - Branch of the ICG SB RAS, 2 Timakova str., 630060 Novosibirsk, Russian Federation; Novosibirsk State University, 2 Pirogova str., 630090 Novosibirsk, Russian Federation; Nikolaev Institute of Inorganic Chemistry SB RAS, 3 Acad. Lavrentieva ave., 630090 Novosibirsk, Russian Federation.
⁵ Scientific Institute of Clinical and Experimental Lymphology - Branch of the ICG SB RAS, 2 Timakova str., 630060 Novosibirsk, Russian Federation; Novosibirsk State University, 2 Pirogova str., 630090 Novosibirsk, Russian Federation.
⁶ Scientific Institute of Clinical and Experimental Lymphology - Branch of the ICG SB RAS, 2 Timakova str., 630060 Novosibirsk, Russian Federation; Scientific Institute of Biochemistry, 2 Timakova str., 630060 Novosibirsk, Russian Federation.

PMID: 30573238
DOI: 10.1016/j.msec.2018.10.084

Abstract

The present work reports ultra-small polyelectrolyte-coated water insoluble Tb(III) complex species with bright Tb(III)-centered luminescence resulted from efficient ligand-to-metal energy transfer as efficient labels for Hep-2 cells. The flow cytometry data revealed the enhanced cellular uptake of negatively charged nanoparticles coated by the polystyrenesulfonate (PSS)-monolayer versus the positively charged nanoparticles. The latter are obtained by layer-by-layer deposition of polyethyleneimine (PEI) onto PSS-coated ones. Confocal and TEM images of Hep-2 cells exposed by the colloids confirm favorable cell internalization of the PSS- compared to PEI-PSS-coated colloids illustrating unusual charge-effect. Dynamic light scattering data indicate significant effect of the biological background exemplified by serum bovine albumin and phosphatidylcholine-based bilayers on the exterior charge and aggregation behavior of the colloids. The obtained results reveal the PSS-coated nanoparticles based on water insoluble Tb(III) complex as promising cell labels.

Keywords: Cell label; Cellular uptake; Layer-by-layer; Polyelectrolyte; Tb(III) chelate nanoparticles.

MeSH terms

Cell Line, Tumor
Flow Cytometry
Hep G2 Cells
Humans
Luminescence
Microscopy, Confocal
Microscopy, Electron, Transmission
Nanoparticles / chemistry*
Polyelectrolytes / chemistry*

Substances

Polyelectrolytes