Biofilms are ubiquitous in nature found on nearly every type of living and inert surface. They basically consist of microorganisms attached to surfaces and surrounded by a self-produced matrix of extracellular polymeric substances. Phages have proven to be successful in controlling biofilms. Here, we describe methods to characterize phage-biofilm interactions, specifically to assess biofilm biomass and to visualize the biofilm structure, discriminating infected cells using targeted molecular probes.
Keywords: Bacteria; Bacteriophage; Biofilm; Control; Microscopy.