An SPR Sensor Chip Based on Peptide-Modified Single-Walled Carbon Nanotubes with Enhanced Sensitivity and Selectivity in the Detection of 2,4,6-Trinitrotoluene Explosives

Jin Wang; Sanyang Du; Takeshi Onodera; Rui Yatabe; Masayoshi Tanaka; Mina Okochi; Kiyoshi Toko

doi:10.3390/s18124461

An SPR Sensor Chip Based on Peptide-Modified Single-Walled Carbon Nanotubes with Enhanced Sensitivity and Selectivity in the Detection of 2,4,6-Trinitrotoluene Explosives

Sensors (Basel). 2018 Dec 17;18(12):4461. doi: 10.3390/s18124461.

Authors

Jin Wang^{1

2}, Sanyang Du^{3

4}, Takeshi Onodera^{5

6}, Rui Yatabe^{7

8}, Masayoshi Tanaka^{9

10}, Mina Okochi^{11

12}, Kiyoshi Toko^{13

14

15}

Affiliations

¹ Research and Development Center for Five-Sense Devices, Kyushu University, 744 Motooka, Nishiku, Fukuoka 819-0395, Japan. wang.jin.335@m.kyushu-u.ac.jp.
² JST, ImPACT, Sanban-cho 5, Chiyoda-ku, Tokyo 102-0075, Japan. wang.jin.335@m.kyushu-u.ac.jp.
³ JST, ImPACT, Sanban-cho 5, Chiyoda-ku, Tokyo 102-0075, Japan. du.sanyang.748@s.kyushu-u.ac.jp.
⁴ Graduate School of Information Science and Electrical Engineering, Kyushu University, 744 Motooka, Nishiku, Fukuoka 819-0395, Japan. du.sanyang.748@s.kyushu-u.ac.jp.
⁵ JST, ImPACT, Sanban-cho 5, Chiyoda-ku, Tokyo 102-0075, Japan. onodera@ed.kyushu-u.ac.jp.
⁶ Graduate School of Information Science and Electrical Engineering, Kyushu University, 744 Motooka, Nishiku, Fukuoka 819-0395, Japan. onodera@ed.kyushu-u.ac.jp.
⁷ JST, ImPACT, Sanban-cho 5, Chiyoda-ku, Tokyo 102-0075, Japan. yatabe.rui.678@m.kyushu-u.ac.jp.
⁸ Graduate School of Information Science and Electrical Engineering, Kyushu University, 744 Motooka, Nishiku, Fukuoka 819-0395, Japan. yatabe.rui.678@m.kyushu-u.ac.jp.
⁹ JST, ImPACT, Sanban-cho 5, Chiyoda-ku, Tokyo 102-0075, Japan. m_tanaka@chemeng.titech.ac.jp.
¹⁰ Department of Chemical Science and Engineering, Tokyo Institute of Technology, 2-12-1, O-okayama, Meguro-ku, Tokyo 152-8552, Japan. m_tanaka@chemeng.titech.ac.jp.
¹¹ JST, ImPACT, Sanban-cho 5, Chiyoda-ku, Tokyo 102-0075, Japan. okochi@chemeng.titech.ac.jp.
¹² Department of Chemical Science and Engineering, Tokyo Institute of Technology, 2-12-1, O-okayama, Meguro-ku, Tokyo 152-8552, Japan. okochi@chemeng.titech.ac.jp.
¹³ Research and Development Center for Five-Sense Devices, Kyushu University, 744 Motooka, Nishiku, Fukuoka 819-0395, Japan. toko@ed.kyushu-u.ac.jp.
¹⁴ JST, ImPACT, Sanban-cho 5, Chiyoda-ku, Tokyo 102-0075, Japan. toko@ed.kyushu-u.ac.jp.
¹⁵ Institute for Advanced Study, Kyushu University, 744 Motooka, Nishiku, Fukuoka 819-0395, Japan. toko@ed.kyushu-u.ac.jp.

Abstract

In this study, we developed a surface plasmon resonance (SPR) sensor chip based on 2,4,6-trinitrotoluene (TNT) recognition peptide-modified single-walled carbon nanotubes (SWCNTs). The carboxylic acid-functionalized SWCNTs were immobilized on a 3-aminopropyltriethoxysilane (APTES)-modified SPR Au chip surface. Through π-stacking between the aromatic amino acids and SWCNTs, the TNT recognition peptide TNTHCDR3 was immobilized onto the surface of the SWCNTs. The peptide⁻SWCNTs-modified sensor surface was confirmed and evaluated by atomic force microscope (AFM) observation. The peptide⁻SWCNTs hybrid SPR sensor chip exhibited enhanced sensitivity with a limit of detection (LOD) of 772 ppb and highly selective detection compared with commercialized carboxymethylated dextran matrix sensor chips.

Keywords: SPR; SWCNTs; TNT analogues; TNTHCDR3; π-stacking.

Grants and funding

HQJJ268702/Japan Science and Technology Agency