Characterization of a novel MICB variant in an individual from the Chinese Uyghur population, MICB*005:09, by cloning and sequencing

Abstract

A new allelic variant in MICB*005 lineage, MICB*005:09, has been identified in a male Uyghur individual recruited from Xinjiang Uyghur Autonomous Region, China by PCR-sequence-based typing (Sanger sequencing) and confirmed by cloning and sequencing. Aligned with MICB*005:03, this new allelic variant shows a synonymous T substitution at nucleotide position 8 in exon 2, corresponding to codon 3 (CAC→CAT) of the mature MICB mRNA transcript.

Keywords: MICB*005:09; Chinese Uyghur population; Sanger sequencing; cloning.