Proteoglycan-substrate gel zymography for the detection of chondroitin sulfate-degrading enzymes

Anal Biochem. 2019 Mar 1:568:51-52. doi: 10.1016/j.ab.2018.12.010. Epub 2018 Dec 14.

Abstract

Chondroitin sulfate (CS), a linear polysaccharide, is a major component of the cartilage matrix. Although CS plays various roles in several biological and pathological processes, most details regarding its metabolism are still poorly understood. Some CS-degrading enzymes have been identified in mammals, but their expression patterns and localizations remain unclear. Here we present a simple zymography procedure to detect CS-degrading enzymes using salmon nasal cartilage proteoglycans as substrates. This method should be useful to explore CS-degrading enzymes.

Keywords: Chondroitin sulfate; Chondroitinase; Hyaluronidase; Proteoglycan; Zymography.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chondroitin Sulfates / chemistry*
  • Chondroitin Sulfates / metabolism*
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme Assays / instrumentation*
  • Enzyme Assays / methods*
  • Gels / chemistry
  • Hyaluronoglucosaminidase / analysis*
  • Hyaluronoglucosaminidase / metabolism
  • Peptide Hydrolases / analysis*
  • Peptide Hydrolases / metabolism
  • Proteoglycans / chemistry*
  • Salmon

Substances

  • Gels
  • Proteoglycans
  • Chondroitin Sulfates
  • Hyaluronoglucosaminidase
  • Peptide Hydrolases