Three alkaloids including a new one, N-formyldihydrochelerythrine (1), together with four other known compounds were isolated from the stem bark of Caloncoba glauca. The structure of the new compound was elucidated from spectroscopic and mass spectrometric evidence. This is the first report of alkaloids from the genus Caloncoba. Sesamin (4) [MIC = 256 μg/mL (Candida albicans) and dihydrochelerythrine (2) [MIC = 32 (C. albicans and C. parapsilosis), and 128 μg/mL (C. krusei)] had moderate to weak antifungal activity.