Nocardioides gansuensis sp. nov., isolated from geopark soil

Abstract

A novel bacterial strain, designated WSJ-1^T, was isolated from geopark soil in Gansu province, PR China. The highest 16S rRNA gene sequence similarities were to those of Nocardioides sediminis MSL-01^T (97.10 %), Nocardioides aquiterrae GW-9^T (97.08 %) and Nocardioides terrigena DS-17^T (96.94 %). Strain WSJ-1^T grouped with N. terrigena DS-17^T and N. sediminis MSL-01^T in 16S rRNA gene-based phylogenetic trees. The DNA-DNA relatedness of WSJ-1^T/N. sediminis JCM19559^T and WSJ-1^T/N. aquiterrae JCM11813^T were 44.8 and 29.2 %, respectively. Average nucleotide identity values of whole genome sequences of WSJ-1^T/N. terrigena KCTC19217^T and WSJ-1^T/N. sediminis KCTC19271^T were 78.83 and 78.83 %, respectively. Its genome size was 4.76 Mb, comprising 4517 predicted genes with a DNA G+C content of 70.9 %. Strain WSJ-1^T contained menaquinone-8(H4) as the major respiratory quinone and ll-2,6-diaminopimelic acid as the diagnostic diamino acid in the cell-wall peptidoglycan. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol and phosphatidylinositol. The major cellular fatty acids were iso-C16 : 0, C18 : 1ω9c and C17 : 1ω8c. Based on the polyphasic analyses, the isolate is considered to represent a novel species of the genus Nocardioides, for which the name Nocardioidesgansuensis sp. nov. is proposed. The type strain is WSJ-1^T (=KCTC 49117^T=CCTCC AB 2018027^T).

Keywords: Nocardioidaceae; Nocardioides gansuensis; geopark soil.