Nanoparticles possess a number of useful properties that make them useful for a variety of industrial and commercial applications. The small size of nanoparticles means that they are respirable and can penetrate deep into the lung when inhaled. Because of this, there is interest in assessing possible toxic effects of nanoparticles on the respiratory system. Measurement of respiratory mechanics and pulmonary function represents a sensitive way of detecting pathological effects of inhaled substances on the lungs. Here we describe a procedure for conducting pulmonary function measurements in mice using the forced oscillation technique. Measurements of baseline lung mechanics are conducted in anesthetized, mechanically ventilated mice, followed by repeated measurements subsequent to inhalation challenge with aerosolized methacholine. General guidelines for data analysis are provided, and sample results are presented.
Keywords: Forced oscillation technique; Lung compliance; Lung resistance; Pulmonary function testing.