Complete Nucleotide Sequence of a Partitivirus from Rhizoctonia solani AG-1 IA Strain C24

Viruses. 2018 Dec 11;10(12):703. doi: 10.3390/v10120703.

Abstract

The complete genome of a novel double-stranded (ds) RNA mycovirus, named as Rhizoctonia solani partitivirus 5 (RsPV5), isolated from rice sheath blight fungus R. solani AG-1 IA strain C24, was sequenced and analysed. RsPV5 consists of two segments, dsRNA-1 (1899 nucleotides) and dsRNA-2 (1787 nucleotides). DsRNA-1 has an open reading frame (ORF) 1 that potentially codes for a protein of 584 amino acid (aa) containing the conserved motifs of a RNA-dependent RNA polymerase (RdRp), and dsRNA-2 also contains a ORF 2, encoding a putative capsid protein (CP) of 513 aa. Phylogenetic analysis revealed that RsPV5 clustered together with six other viruses in an independent clade of the genus Alphapartitivirus, indicating that RsPV5 was a new member of the genus Alphapartitivirus, within the family Partitiviridae.

Keywords: Alphapartitivirus; Rhizoctonia solani AG-1 IA; dsRNA; genomic structure analysis; mycovirus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fungal Viruses / genetics*
  • Fungal Viruses / isolation & purification
  • Genome, Viral*
  • Open Reading Frames
  • Oryza / microbiology
  • Phylogeny
  • Plant Diseases / microbiology
  • RNA, Double-Stranded / genetics
  • RNA, Viral / genetics*
  • RNA-Dependent RNA Polymerase / genetics
  • Rhizoctonia / virology*
  • Sequence Analysis, DNA

Substances

  • RNA, Double-Stranded
  • RNA, Viral
  • RNA-Dependent RNA Polymerase