Enhancement of drug electrotransfer by extracellular plasmid DNA

Paulius Ruzgys; Milda Jakutaviciute; Sonam Chopra; Saulius Satkauskas

doi:10.1016/j.abb.2018.12.010

Enhancement of drug electrotransfer by extracellular plasmid DNA

Arch Biochem Biophys. 2019 May 15:666:156-160. doi: 10.1016/j.abb.2018.12.010. Epub 2018 Dec 10.

Authors

Paulius Ruzgys¹, Milda Jakutaviciute¹, Sonam Chopra¹, Saulius Satkauskas²

Affiliations

¹ Biophysical Research Group, Vytautas Magnus University, Kaunas, Lithuania.
² Biophysical Research Group, Vytautas Magnus University, Kaunas, Lithuania. Electronic address: saulius.satkauskas@vdu.lt.

PMID: 30543787
DOI: 10.1016/j.abb.2018.12.010

Abstract

Electroporation is a widely established method for molecular delivery across electric field perturbed plasma membrane. It can be used as a non-viral DNA transfection method, or as a way to achieve small molecule delivery to or extraction from cells. We examined the possibility of combining the DNA delivery to the cells with small molecule transport across electroporated plasma membrane. The results show that the presence of DNA in electroporation medium increases the extraction of fluorescent dye calcein from calcein-AM loaded cells as well as the delivery of small-molecule drug bleomycin to the cells. We propose that these results may have implications in enhanced drug delivery using electroporation both in vivo and in clinics.

Keywords: Bleomycin; Calcein; DNA electrotransfer; Drug delivery; Electroporation; Plasmid DNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bleomycin / administration & dosage
Cell Membrane / metabolism
DNA / administration & dosage*
Electroporation / methods*
Fluoresceins / chemistry
Fluorescent Dyes / chemistry
Plasmids*
Transfection

Substances

Fluoresceins
Fluorescent Dyes
Bleomycin
DNA
fluorexon