Abstract
Two new isochromanone derivatives, (3S,4S)-3,8-dihydroxy-6-methoxy-3,4,5-trimethylisochroman-1-one (1) and methyl (S)-8-hydroxy-6-methoxy-5-methyl-4a-(3-oxobutan-2-yl)benzoate (2), together with six known compounds (3‒8) were isolated from the cultures of an endophytic fungus Phoma sp. PF2 obtained from Artemisia princeps. The chemical structures of the isolated compounds were elucidated by interpretation of spectroscopic data (1D, 2D NMR, HRESIMS, and CD) and calculation of ECD. All the isolated compounds (1‒8) showed moderate inhibitory activities on nitric oxide levels in lipopolysaccharide-induced RAW264.7 machrophage cells.
MeSH terms
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Animals
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Artemisia / microbiology*
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Ascomycota / growth & development
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Ascomycota / isolation & purification
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Ascomycota / metabolism*
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Biological Products / chemistry
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Biological Products / isolation & purification*
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Biological Products / pharmacology*
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Circular Dichroism
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Culture Media / chemistry
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Endophytes / growth & development
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Endophytes / isolation & purification
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Endophytes / metabolism*
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Immunosuppressive Agents / chemistry
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Immunosuppressive Agents / isolation & purification*
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Immunosuppressive Agents / pharmacology*
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Magnetic Resonance Spectroscopy
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Mice
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Molecular Structure
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RAW 264.7 Cells
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Spectrometry, Mass, Electrospray Ionization
Substances
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Biological Products
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Culture Media
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Immunosuppressive Agents