Biomimetic recyclable microgels for on-demand generation of hydrogen peroxide and antipathogenic application

Abstract

Microgels that can generate antipathogenic levels of hydrogen peroxide (H₂O₂) through simple rehydration in solutions with physiological pH are described herein. H₂O₂ is a widely used disinfectant but the oxidant is hazardous to store and transport. Catechol, an adhesive moiety found in mussel adhesive proteins, was incorporated into microgels, which generated 1-5 mM of H₂O₂ for up to four days as catechol autoxidized. The sustained release of low concentrations of H₂O₂ was antimicrobial against both gram-positive (Staphylococcus epidermidis) and gram-negative (Escherichia coli) bacteria and antiviral against both non-enveloped porcine parvovirus (PPV) and enveloped bovine viral diarrhea virus (BVDV). The amount of released H₂O₂ is several orders of magnitude lower than H₂O₂ concentration previously reported for antipathogenic activity. Most notably, these microgels reduced the infectivity of the more biocide resistant non-envelope virus by 3 log reduction value (99.9% reduction in infectivity). By controlling the oxidation state of catechol, microgels can be repeatedly activated and deactivated for H₂O₂ generation. These microgels do not contain a reservoir for storing the reactive H₂O₂ and can potentially function as a lightweight and portable dried powder source for the disinfectant for a wide range of applications. STATEMENT OF SIGNIFICANCE: Researchers have designed bioadhesives and coatings using the adhesive moiety catechol to mimic the strong adhesion capability of mussel adhesive proteins. During catechol autoxidation, hydrogen peroxide (H₂O₂) is generated as a byproduct. Here, catechol was incorporated into microgels, which can generate millimolar levels of H₂O₂ by simply hydrating the microgels in a solution with physiological pH. The sustained release of H₂O₂ was both antimicrobial and antiviral, inactivating even the more biocide resistant non-enveloped virus. These microgels can be repeatedly activated and deactivated for H₂O₂ generation by incubating them in solutions with different pH. This simplicity and recyclability will enable this biomaterial to function as a lightweight and portable source for the disinfectant for a wide range of applications.

Keywords: Antimicrobial; Antiviral; Dopamine; Hydrogen peroxide; Recyclable microgels.