Routine use of MALDI-TOF MS for anaerobic bacterial identification in clinical microbiology

Anaerobe. 2018 Dec:54:191-196. doi: 10.1016/j.anaerobe.2018.07.001. Epub 2018 Jul 3.

Abstract

In 2013, we adopted MALDI-TOF MS using the Bruker Biotyper system for identification of anaerobic bacteria into our routine clinical practice. Here, we describe our experience with the use of MALDI-TOF MS for anaerobic bacterial identification, highlighting its value in replacing the more costly and time-consuming 16S ribosomal RNA gene PCR plus sequencing-based approach as the primary method of anaerobic bacterial identification. We also describe our more recent experience with the use of early/rapid MALDI-TOF MS for identification of anaerobic bacteria performed on short incubation (4-6 h) plated aerobic media from anaerobic blood culture bottles positive for Gram-negative bacilli.

Keywords: 16S rRNA gene PCR/sequencing; Anaerobe; Bruker; Early MALDI; MALDI-TOF MS; Rapid MALDI.

Publication types

  • Evaluation Study

MeSH terms

  • Bacteria, Anaerobic / chemistry
  • Bacteria, Anaerobic / classification
  • Bacteria, Anaerobic / genetics
  • Bacteria, Anaerobic / isolation & purification*
  • Bacterial Infections / diagnosis
  • Bacterial Infections / microbiology*
  • Bacterial Typing Techniques / methods*
  • DNA, Bacterial / genetics
  • Diagnostic Tests, Routine / methods*
  • Gram-Negative Bacteria / chemistry
  • Gram-Negative Bacteria / classification
  • Gram-Negative Bacteria / genetics
  • Gram-Negative Bacteria / isolation & purification
  • Humans
  • Polymerase Chain Reaction
  • RNA, Ribosomal, 16S / genetics
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

  • DNA, Bacterial
  • RNA, Ribosomal, 16S