Determination of Anti-aquaporin 5 Autoantibodies by Immunofluorescence Cytochemistry

Jehan Alam; Sumin Jeon; Youngnim Choi

doi:10.1007/978-1-4939-8949-2_6

Determination of Anti-aquaporin 5 Autoantibodies by Immunofluorescence Cytochemistry

Methods Mol Biol. 2019:1901:79-87. doi: 10.1007/978-1-4939-8949-2_6.

Authors

Jehan Alam^{1

2}, Sumin Jeon¹, Youngnim Choi³

Affiliations

¹ Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
² Mary and Dick Holland Regenerative Medicine Program, University of Nebraska Medical Center, Omaha, NE, USA.
³ Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Republic of Korea. youngnim@snu.ac.kr.

PMID: 30539569
DOI: 10.1007/978-1-4939-8949-2_6

Abstract

Cell-based assay by immunofluorescence cytochemistry (CBA-IFC) has been shown to be the most accurate method to detect anti-aquaporin (AQP) 4 autoantibodies. Detection of anti-AQP5 autoantibodies is delicate, which depends on the proper expression of AQP5 on the plasma membrane. Here, we describe methods to determine anti-AQP5 autoantibodies by CBA-IFC. Both anti-AQP5 IgG and IgA can be detected by this method.

Keywords: Aquaporin 5; Autoantibodies; Cell-based assay; Immunofluorescence cytochemistry; Plasma membrane.

MeSH terms

Animals
Antigens / metabolism
Aquaporin 5 / immunology*
Autoantibodies / analysis*
Dogs
Fluorescent Antibody Technique / methods*
Humans
Madin Darby Canine Kidney Cells
Microscopy, Fluorescence
ROC Curve
Staining and Labeling

Substances

Antigens
Aquaporin 5
Autoantibodies