Blood Plasma Separation Using a Fidget-Spinner

Anal Chem. 2019 Jan 15;91(2):1247-1253. doi: 10.1021/acs.analchem.8b04860. Epub 2018 Dec 26.

Abstract

In this study, we present a simple, hand-powered, and electricity-free centrifuge platform based on a commercially available "fidget-spinner." The centrifugal force provided by this inexpensive and easy-to-use toy is sufficient to separate whole blood, producing a plasma yield rate and purity of 30% and 99%, respectively, separated in as little as 4-7 min. We verified the separated plasma by performing a paper-based HIV-1 p24 capsid protein enzyme-linked immunosorbent assay, which achieved a recovery rate of up to 98%, indicating the plasma features extremely low matrix interference effects. These results demonstrate the reliability of the platform for practical use, in addition to greatly reducing the overall cost and time of analysis while retaining detection precision, making it suitable for medical applications in resource-limited regions of the world.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies / immunology
  • Cell Separation / instrumentation
  • Cell Separation / methods*
  • Centrifugation / instrumentation
  • Centrifugation / methods
  • Enzyme-Linked Immunosorbent Assay
  • HIV Core Protein p24 / immunology
  • HIV-1 / chemistry
  • Humans
  • Mice
  • Plasma / cytology*
  • Play and Playthings*

Substances

  • Antibodies
  • HIV Core Protein p24
  • p24 protein, Human Immunodeficiency Virus Type 1