Long-Term Culture of Mouse Fetal Hepatic Stem/Progenitor Cells

Atsunori Tsuchiya; Shuji Terai

doi:10.1007/978-1-4939-8961-4_1

Long-Term Culture of Mouse Fetal Hepatic Stem/Progenitor Cells

Methods Mol Biol. 2019:1905:3-8. doi: 10.1007/978-1-4939-8961-4_1.

Authors

Atsunori Tsuchiya¹, Shuji Terai²

Affiliations

¹ Division of Gastroenterology and Hepatology, Graduate School of Medical and Dental Science, Niigata University, Niigata, Japan. atsunori@med.niigata-u.ac.jp.
² Division of Gastroenterology and Hepatology, Graduate School of Medical and Dental Science, Niigata University, Niigata, Japan.

PMID: 30536085
DOI: 10.1007/978-1-4939-8961-4_1

Abstract

Mouse fetal liver includes abundant hepatic stem/progenitor cells (HSPCs). Easy expansion with passage of HSPCs is necessary to obtain steady data. However, it is often difficult to enrich only HSPCs, and HSPCs can die when usual trypsin is used for replating. Here, we introduce serum-free long-term culture with passage of HSPCs using fetal mouse liver without a cell sorter.

Keywords: Colony; Hepatic progenitor cell; Mouse; Serum-free culture; Spheroid.

MeSH terms

Animals
Cell Culture Techniques / methods*
Cell Proliferation / drug effects
Cell Survival
Cells, Cultured
Culture Media, Serum-Free / pharmacology
Female
Fetal Stem Cells / cytology*
Fetal Stem Cells / drug effects
Flow Cytometry
Liver / cytology
Liver / embryology*
Mice
Pregnancy

Substances

Culture Media, Serum-Free