Genomic Alterations of Staphylococcus aureus ATCC 25923 after Prolonged Passage in the Laboratory

Pei-Ju Chin; Hsiao-Mei Liao; Bingjie Li; Guo-Chiuan Hung; Shien Tsai; Shyh-Ching Lo

doi:10.1128/MRA.01108-18

Genomic Alterations of Staphylococcus aureus ATCC 25923 after Prolonged Passage in the Laboratory

Microbiol Resour Announc. 2018 Nov 8;7(18):e01108-18. doi: 10.1128/MRA.01108-18. eCollection 2018 Nov.

Authors

Pei-Ju Chin^#¹, Hsiao-Mei Liao^#¹, Bingjie Li¹, Guo-Chiuan Hung¹, Shien Tsai¹, Shyh-Ching Lo¹

Affiliation

¹ Tissue Microbiology Laboratory, Division of Cellular and Gene Therapies, Office of Tissues and Advanced Therapies, Center for Biologics Evaluation and Research, Food and Drug Administration, Silver Spring, Maryland, USA.

^# Contributed equally.

Abstract

Staphylococcus aureus reference strain ATCC 25923 has been maintained for more than a decade in our laboratory. Genomic study revealed that the resulting strain AFIPCBER_B_8.4 has lost a 37-kb genomic fragment of the ATCC 25923 parental strain. The missing fragment showed sequence similarity to genes of bacteriophage proteins.