An IncR Plasmid Harbored by a Hypervirulent Carbapenem-Resistant Klebsiella pneumoniae Strain Possesses Five Tandem Repeats of the blaKPC-2::NTEKPC-Id Fragment

Ning Dong; Lizhang Liu; Rong Zhang; Kaichao Chen; Miaomiao Xie; Edward Wai Chi Chan; Sheng Chen

doi:10.1128/AAC.01775-18

An IncR Plasmid Harbored by a Hypervirulent Carbapenem-Resistant Klebsiella pneumoniae Strain Possesses Five Tandem Repeats of the bla_KPC-2::NTE_KPC-Id Fragment

Antimicrob Agents Chemother. 2019 Feb 26;63(3):e01775-18. doi: 10.1128/AAC.01775-18. Print 2019 Mar.

Authors

Ning Dong^{1

2}, Lizhang Liu^{1

2}, Rong Zhang³, Kaichao Chen^{1

2}, Miaomiao Xie^{1

2}, Edward Wai Chi Chan^{1

2}, Sheng Chen^{4

2}

Affiliations

¹ Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, People's Republic of China.
² State Key Lab of Chirosciences, Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong.
³ Department of Clinical Laboratory Medicine, Second Affiliated Hospital of Zhejiang University, Hangzhou, People's Republic of China.
⁴ Shenzhen Key Lab for Food Biological Safety Control, Food Safety and Technology Research Center, Hong Kong PolyU Shen Zhen Research Institute, Shenzhen, People's Republic of China sheng.chen@polyu.edu.hk.

Abstract

Completed sequences of three plasmids from a carbapenem-resistant hypervirulent Klebsiella pneumoniae isolate, SH9, were obtained. In addition to the pLVPK-like virulence-conferring plasmid (pVir-CR-HvKP_SH9), the two multidrug-resistant plasmids (pKPC-CR-HvKP4_SH9 and pCTX-M-CR-HvKP4_SH9) were predicted to originate from a single pKPC-CR-HvKP4-like multireplicon plasmid through homologous recombination. Interestingly, the bla_KPC-2 gene was detectable in five tandem repeats exhibiting the format of an NTE_KPC-Id-like transposon (IS26-ΔTn3-ISKpn8-bla_KPC-2-ΔISKpn6-korC-orf-IS26). The data suggest an important role of DNA recombination in mediating active plasmid evolution.

Keywords: Klebsiella pneumoniae; blaKPC-2; cointegration; hypervirulent; tandem repeat.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbapenem-Resistant Enterobacteriaceae / genetics*
Humans
Klebsiella Infections / microbiology
Klebsiella pneumoniae / drug effects*
Klebsiella pneumoniae / genetics*
Klebsiella pneumoniae / pathogenicity
Plasmids / genetics*
Recombination, Genetic / genetics
Tandem Repeat Sequences / genetics
Whole Genome Sequencing
beta-Lactamases / genetics*

Substances

beta-Lactamases
beta-lactamase KPC-2, Klebsiella pneumoniae