Study on MgxSr3-x(PO4)2 bioceramics as potential bone grafts

Colloids Surf B Biointerfaces. 2019 Mar 1:175:158-165. doi: 10.1016/j.colsurfb.2018.11.085. Epub 2018 Nov 30.

Abstract

Magnesium (Mg) and strontium (Sr), which are essential nutrient elements in the natural bone, positively affect the osteogenic activity even in wide ranges of ion concentrations. However, it remains unknown whether magnesium-strontium phosphates [MgxSr3-x(PO4)2] are potential bone grafts for accelerating bone regeneration. Herein, a serial of MgxSr3-x(PO4)2, including Mg3(PO4)2, Mg2Sr(PO4)2, Mg1.5Sr1.5(PO4)2, MgSr2(PO4)2 and Sr3(PO4)2, were synthesized using a solid-state reaction approach. The physicochemical properties and cell behaviors of MgxSr3-x(PO4)2 bioceramics were characterized and compared with the common bone graft β-tricalcium phosphate (β-TCP). The results indicated that various MgxSr3-x(PO4)2 bioceramics differed in compressive strength and in vitro degradation rate. All the MgxSr3-x(PO4)2 bioceramics had excellent biocompatibility. In contrast to β-TCP, the MgxSr3-x(PO4)2 enhanced alkaline phosphatase activity of mouse bone mesenchymal stem cells (mBMSCs), and inhibited osteoclastogenesis-related gene expression of RAW264.7 cells, but did not enhance osteogenesis-related gene expression of mBMSCs which were treated with osteogenesis induction supplements. However, Mg3(PO4)2 stimulated osteogenesis-related gene expression of mBMSCs without the treatment of osteogenesis induction supplements. This work contributes to the design of bone graft and may open a new avenue for the bone regeneration field.

Keywords: Bioceramic; Bone regeneration; Cell response; Magnesium; Strontium.

MeSH terms

  • Animals
  • Biocompatible Materials / chemical synthesis
  • Biocompatible Materials / chemistry
  • Biocompatible Materials / pharmacology*
  • Bone Regeneration / drug effects
  • Bone Regeneration / genetics
  • Bone Substitutes / chemistry
  • Bone Transplantation / methods
  • Bone and Bones / cytology
  • Bone and Bones / drug effects
  • Bone and Bones / metabolism
  • Cell Survival / drug effects
  • Cell Survival / genetics
  • Cells, Cultured
  • Ceramics / chemical synthesis
  • Ceramics / chemistry
  • Ceramics / pharmacology*
  • Gene Expression / drug effects
  • Magnesium Compounds / chemical synthesis
  • Magnesium Compounds / chemistry
  • Magnesium Compounds / pharmacology*
  • Mesenchymal Stem Cells / drug effects
  • Mesenchymal Stem Cells / metabolism
  • Mice
  • Osteogenesis / drug effects
  • Osteogenesis / genetics
  • Phosphates / chemical synthesis
  • Phosphates / chemistry
  • Phosphates / pharmacology*
  • RAW 264.7 Cells
  • Strontium / chemistry
  • Strontium / pharmacology*

Substances

  • Biocompatible Materials
  • Bone Substitutes
  • Magnesium Compounds
  • Phosphates
  • magnesium phosphate
  • strontium phosphate
  • Strontium