Alpha-pinene attenuates UVA-induced photoaging through inhibition of matrix metalloproteinases expression in mouse skin

Abstract

Aim: The present study was designed to examine the role of alpha-pinene (AP) against skin photoaging in UVA-irradiated mice.

Materials and methods: Swiss albino mice were subjected to UVA-irradiation at the rate of 10 J/cm² per day for ten days, totally mouse received 100 J/cm². One hour prior to each UVA-exposure, the mouse skin was topically treated with AP (100 mg kg/b·wt). Biochemical methods were employed to study the status of antioxidant enzymes and lipid peroxidation. Histopathological observations were performed using hematoxylin and eosin (H & E) and Verhoeff van Gieson (VVG) staining in the mouse skin. The inflammatory and apoptotic protein expression was studied by immunohistochemical and Western blot methods. The mRNA expression of matrix metalloproteinases was determined by qRT-PCR and Western blot analysis.

Key findings: We found that AP pretreatment substantially ameliorated UVA-induced depletion of antioxidant enzymes and prevented UVA-induced lipid peroxidation in the mouse skin. Further, AP effectively inhibited UVA-induced activation of pro-angiogenic (iNOS and VEGF), inflammatory proteins (TNF-α, IL-6, and COX-2) expression and prevented the activation of NF-κB p65 in the mouse skin. Additionally, AP inhibited UVA-mediated apoptotic mediators (Bax, Bcl-2, caspase-3 and caspase 9) expression in the mouse skin. Moreover, AP inhibited mRNA expression of matrix metalloproteinases (MMP-13 and MMP-9) and tissue type IV collagenase (MMP-2) expression in the mouse skin. Histological studies showed that AP remarkably prevented the dermal tissue damage in UVA-irradiated mice.

Conclusion: Thus, AP treatment effectively prevented UVA-induced photoaging probably through its antioxidant property.

Keywords: Alpha-pinene; Apoptosis; Inflammation; Photoaging; Ultraviolet-A radiation.