Intracellular delivery of nucleic acid by cell-permeable hPP10 peptide

Yi Ding; Xueli Zhao; Jingping Geng; Xiangli Guo; Jielan Ma; Hu Wang; Changbai Liu

doi:10.1002/jcp.27826

Intracellular delivery of nucleic acid by cell-permeable hPP10 peptide

J Cell Physiol. 2019 Jul;234(7):11670-11678. doi: 10.1002/jcp.27826. Epub 2018 Dec 4.

Authors

Yi Ding^{1

2}, Xueli Zhao^{1

2}, Jingping Geng^{1

2}, Xiangli Guo^{1

2}, Jielan Ma^{1

2}, Hu Wang^{1

2

3}, Changbai Liu^{1

2}

Affiliations

¹ Hubei Key Lab of Tumor Microenvironment and Immunotherapy, China Three Gorges University, Yichang, China.
² Department of Pathology and Immunology, Medical School, China Three Gorges University, Yichang, China.
³ Institute for Cell Engineering, Johns Hopkins University School of Medicine, Baltimore, Maryland.

PMID: 30515802
DOI: 10.1002/jcp.27826

Abstract

Although gene therapy offers hope against incurable diseases, nonreplicating transduction vectors remain lacking. We have previously characterized a cell-penetrating peptide hPP10 for the delivery of various cargoes; however, whether hPP10 can mediate nucleic acid delivery is still unknown. Here, examining via different ways, we demonstrate that hPP10 stably complexes with plasmid DNA (pDNA) and safely mediates nucleic acid transfection. hPP10 can mediate GFP-, dsRed-, and luciferase-expressing plasmids into cells with nearly the same efficiency as commercial transfection reagents Turbofectin or Lipofect. Furthermore, hPP10 can mediate Cre fusion protein delivery and pDNA transfection simultaneously in the Cre/loxp system in vitro. In addition, hPP10 fused with an RNA-binding domain can mediate delivery of small interfering RNA into cells to silence the reporter gene expression. Collectively, our results suggest that hPP10 is an option for nucleic acid delivery with efficiencies similar to that of commercial reagents.

Keywords: cell-penetrating peptides (CPPs); nucleic acid; small interfering RNA (siRNA), transfection.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line, Tumor
Cell-Penetrating Peptides / metabolism*
DNA / administration & dosage
Gene Transfer Techniques*
Green Fluorescent Proteins / metabolism
Humans
Intracellular Space / metabolism*
Nucleic Acids / administration & dosage*
Plasmids / administration & dosage
RNA, Small Interfering / metabolism
Transfection

Substances

Cell-Penetrating Peptides
Nucleic Acids
RNA, Small Interfering
Green Fluorescent Proteins
DNA