An intravascular magnetic wire for the high-throughput retrieval of circulating tumour cells in vivo

Ophir Vermesh; Amin Aalipour; T Jessie Ge; Yamil Saenz; Yue Guo; Israt S Alam; Seung-Min Park; Charlie N Adelson; Yoshiaki Mitsutake; Jose Vilches-Moure; Elias Godoy; Michael H Bachmann; Chin Chun Ooi; Jennifer K Lyons; Kerstin Mueller; Hamed Arami; Alfredo Green; Edward I Solomon; Shan X Wang; Sanjiv S Gambhir

doi:10.1038/s41551-018-0257-3

An intravascular magnetic wire for the high-throughput retrieval of circulating tumour cells in vivo

Nat Biomed Eng. 2018 Sep;2(9):696-705. doi: 10.1038/s41551-018-0257-3. Epub 2018 Jul 16.

Authors

Ophir Vermesh^{1

2}, Amin Aalipour^{1

2

3}, T Jessie Ge^{1

2

4}, Yamil Saenz², Yue Guo⁵, Israt S Alam^{1

2}, Seung-Min Park^{1

2}, Charlie N Adelson⁶, Yoshiaki Mitsutake⁷, Jose Vilches-Moure⁸, Elias Godoy⁸, Michael H Bachmann^{2

9}, Chin Chun Ooi¹⁰, Jennifer K Lyons⁶, Kerstin Mueller², Hamed Arami^{1

2}, Alfredo Green⁷, Edward I Solomon⁶, Shan X Wang^{5

11}, Sanjiv S Gambhir^{12

13}

Affiliations

¹ Molecular Imaging Program at Stanford, Stanford University, Stanford, CA, USA.
² Department of Radiology, Stanford University School of Medicine, Stanford, CA, USA.
³ Department of Bioengineering, Stanford University, Stanford, CA, USA.
⁴ Howard Hughes Medical Institute, Chevy Chase, MD, USA.
⁵ Department of Electrical Engineering, Stanford University, Stanford, CA, USA.
⁶ Department of Chemistry, Stanford University, Stanford, CA, USA.
⁷ Division of Cardiovascular Medicine, Stanford University, Stanford, CA, USA.
⁸ Department of Comparative Medicine, Stanford University, Stanford, CA, USA.
⁹ Department of Pediatrics, Stanford University, Stanford, CA, USA.
¹⁰ Department of Chemical Engineering, Stanford University, Stanford, CA, USA.
¹¹ Department of Materials Science and Engineering, Stanford University, Stanford, CA, USA.
¹² Molecular Imaging Program at Stanford, Stanford University, Stanford, CA, USA. sgambhir@stanford.edu.
¹³ Department of Radiology, Stanford University School of Medicine, Stanford, CA, USA. sgambhir@stanford.edu.

Abstract

The detection and analysis of rare blood biomarkers is necessary for early diagnosis of cancer and to facilitate the development of tailored therapies. However, current methods for the isolation of circulating tumour cells (CTCs) or nucleic acids present in a standard clinical sample of only 5-10 ml of blood provide inadequate yields for early cancer detection and comprehensive molecular profiling. Here, we report the development of a flexible magnetic wire that can retrieve rare biomarkers from the subject's blood in vivo at a much higher yield. The wire is inserted and removed through a standard intravenous catheter and captures biomarkers that have been previously labelled with injected magnetic particles. In a proof-of-concept experiment in a live porcine model, we demonstrate the in vivo labelling and single-pass capture of viable model CTCs in less than 10 s. The wire achieves capture efficiencies that correspond to enrichments of 10-80 times the amount of CTCs in a 5-ml blood draw, and 500-5,000 times the enrichments achieved using the commercially available Gilupi CellCollector.

Associated data

figshare/10.6084/m9.figshare.6272414

Abstract

Associated data

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