Exploration of Adhesion Molecule Expression in Cardiac Muscle of Early Atherosclerosis Dyslipidemic Sprague Dawley Rats

Titin A Wihastuti; Fitria N Aini; Nurul C Lutfiana; Teuku Heriansyah; Nafisatuz Zamrudah

doi:10.2174/1874104501812010124

Exploration of Adhesion Molecule Expression in Cardiac Muscle of Early Atherosclerosis Dyslipidemic Sprague Dawley Rats

Open Med Chem J. 2018 Oct 24:12:124-129. doi: 10.2174/1874104501812010124. eCollection 2018.

Authors

Titin A Wihastuti¹, Fitria N Aini¹, Nurul C Lutfiana¹, Teuku Heriansyah², Nafisatuz Zamrudah¹

Affiliations

¹ Departement of Biomedicine, Faculty of Medicine, Brawijaya University, Malang, Indonesia.
² Departement of Cardiology, Faculty of Medicine, Syiah Kuala University, Aceh, Indonesia.

Abstract

Objective: This study is aimed to examine the expression of ICAM-1 and VCAM-1 in cardiac tissue of dyslipidemic Sprague Dawley rats.

Methods: Eight Sprague Dawley strain rats, with 150-200 gram body weight, were divided into two groups. The control group was fed a standard diet, the positive control group was fed a high-fat diet as our previous study for 8 weeks. The pattern of distribution of ICAM-1 and VCAM-1 in cardiac muscle cell was examined by immunofluorescence and observed with a confocal laser scanning microscope. Lipid profile was also examined at the end of the study.

Result: Independent t-test showed no differences in ICAM-1 and VCAM-1 expression in cardiac muscle of hypercholesterol-diet-fed Sprague Dawley rat compared to control.

Conclusion: The expression of ICAM-1 and VCAM-1 in cardiac muscle did not change after the onset of atherosclerosis.

Keywords: Adhesion; Atherosclerosis; Dyslipidemia; ICAM-1; Immunofluorescence; Molecule expression; VCAM-1.