Silver enhanced nano-gold dot blot immunoassay for leptospirosis

Veerapandian Raja; Muthu Prasad; Palanisamy Bothammal; Perumal Saranya; Krishnamoorthi Sumaiya; Charles Solomon Akino Mercy; Kalimuthusamy Natarajaseenivasan

doi:10.1016/j.mimet.2018.11.021

Silver enhanced nano-gold dot blot immunoassay for leptospirosis

J Microbiol Methods. 2019 Jan:156:20-22. doi: 10.1016/j.mimet.2018.11.021. Epub 2018 Nov 28.

Authors

Veerapandian Raja¹, Muthu Prasad¹, Palanisamy Bothammal¹, Perumal Saranya¹, Krishnamoorthi Sumaiya¹, Charles Solomon Akino Mercy¹, Kalimuthusamy Natarajaseenivasan²

Affiliations

¹ Medical Microbiology Laboratory, Department of Microbiology, Center for Excellence in Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
² Medical Microbiology Laboratory, Department of Microbiology, Center for Excellence in Life Sciences, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India. Electronic address: natarajaseenivasan@rediffmail.com.

PMID: 30502367
DOI: 10.1016/j.mimet.2018.11.021

Abstract

Leptospirosis is a widespread zoonotic disease and lacks in efficient diagnostic tools. In the present study, a nanogold based dot blot immunoassay was developed and evaluated for the detection of leptospirosis in human urine samples. This method was found to be rapid (<4 h) with higher sensitivity (>4.2-14.6%) than horse radish peroxidase (HRP) conjugated dot blot assay.

Keywords: Diagnosis; Dot blot; Leptospirosis; Nanogold; Recombinant protein.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins* / blood
Bacterial Proteins* / urine
Gold
Humans
Immunoblotting / methods*
Immunologic Tests / methods*
Leptospira / isolation & purification*
Leptospirosis / diagnosis*
Metal Nanoparticles

Substances

Bacterial Proteins
Gold