High hydrostatic pressure treatments trigger de novo carotenoid biosynthesis in papaya fruit (Carica papaya cv. Maradol)

Abstract

High hydrostatic pressure (HHP) processing is a non-thermal technology reported to increase desirable metabolites in plant foods. This work evaluated changes in carotenoid accumulation in fresh-cut papaya fruit as affected by HHP treatment (50-400 MPa for 3-60 min) and during subsequent storage at 4 °C; simultaneously, transcriptional activities of carotenoid biosynthetic genes and oxidative stress markers were evaluated. LC-MS analyses revealed that HHP treatment increased carotenoid precursors and carotenes contents following processing and storage: lycopene levels increased up to 11-fold compared to the non-treated samples, and H₂O₂ and lipid peroxidation were concomitantly increased. qRT-PCR of intact RNA showed that the amount of phytoene desaturase transcripts increased after HHP treatment, and that they were correlated with carotene accumulation. This is the first study to show that HHP treatment triggers de novo carotenoid biosynthesis, which is regulated at the transcriptional level, possibly by inducing oxidative stress signaling in fruit tissue.

Keywords: 15-cis-Phytoene (PubChem CID: 5280784); All-trans-lycopene (PubChem CID: 446925); Beta-carotene (PubChem CID: 5280489); Beta-cryptoxanthin (PubChem CID 5281235); Carotenoid biosynthesis; High hydrostatic pressure; Papaya fruit; Phytoene desaturase; Phytofluene (PubChem CID: 6436722); Transcriptional regulation; Zeinoxanthin (PubChem CID: 5281234); Zeta-carotene (PubChem CID: 5280788).