An electric potential modulated cascade of catalyzed hairpin assembly and rolling chain amplification for microRNA detection

Shuang Wang; Shasha Lu; Jiahui Zhao; Jing Ye; Jianshe Huang; Xiurong Yang

doi:10.1016/j.bios.2018.09.088

An electric potential modulated cascade of catalyzed hairpin assembly and rolling chain amplification for microRNA detection

Biosens Bioelectron. 2019 Feb 1:126:565-571. doi: 10.1016/j.bios.2018.09.088. Epub 2018 Sep 28.

Authors

Shuang Wang¹, Shasha Lu¹, Jiahui Zhao², Jing Ye³, Jianshe Huang⁴, Xiurong Yang⁵

Affiliations

¹ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun, Jilin 130022, China; University of Science and Technology of China, Hefei, Anhui 230026, China.
² State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun, Jilin 130022, China; University of Chinese Academy of Sciences, Beijing 100039, China.
³ University of Science and Technology of China, Hefei, Anhui 230026, China.
⁴ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun, Jilin 130022, China. Electronic address: huangjs@ciac.ac.cn.
⁵ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun, Jilin 130022, China; University of Science and Technology of China, Hefei, Anhui 230026, China; University of Chinese Academy of Sciences, Beijing 100039, China. Electronic address: xryang@ciac.ac.cn.

PMID: 30500771
DOI: 10.1016/j.bios.2018.09.088

Abstract

MicroRNAs serve as a new type of biomarker for multifarious diseases due to its critical roles in post transcriptional gene regulation. Herein, we firstly integrate the catalyzed hairpin assembly (CHA) and rolling circle amplification (RCA) into an electrochemical biosensor for sensitive and specific detection of miR-21. Meanwhile, an electric potential was employed to modulate the efficiency of CHA occurred on the electrode, which offer a simple but effective method to surmount the accessibility problem of probes. The biosensor achieved an ultrasensitive determination of miR-21 with a low limit of detection of 13.5 fM and a linear range from 15 fM to 250 pM. This research encourages us to challenge the hyphenated multiple amplification strategies and provides a stable and effective method for the detection of diseases-related miRNAs in peripheral biofluids, as well as paves a road for the future clinical diagnostics and treatment of disease.

Keywords: Cascade amplification; Catalyzed hairpin assembly; Electric potential; G-quadruplex; MiRNA; Rolling circle amplification.

MeSH terms

Biosensing Techniques*
Catalysis
DNA, Catalytic / chemistry
DNA, Catalytic / genetics
Electrodes
Gene Expression Regulation
Humans
Limit of Detection
MicroRNAs / chemistry
MicroRNAs / isolation & purification*
RNA, Small Interfering / chemistry*

Substances

DNA, Catalytic
MIRN21 microRNA, human
MicroRNAs
RNA, Small Interfering