Abstract
We studied the influence of magnetite nanoparticles (FeO•Fe2O3) and quantum dots (CdSe/ZnS coated with mercaptopropionic acid) on the expression of 5 common reference genes (BA, B2M, PPIA, UBC, and YWHAZ) in peripheral blood cells from 20 volunteers by reverse transcription PCR method. The stability of the expression of reference genes varied depending of the cells type and chemical structure of nanoparticles. The level of YWHAZ mRNA after exposure by nanoparticles demonstrated highest stability in lymphocytes, neutrophils, and monocytes. Stability of YWHAZ expression was confirmed by Western blotting. Our findings suggest that YWHAZ is the most suitable as the reference gene.
Keywords:
leukocytes; nanoparticles; peripheral blood; quantum dots; reference gene.
MeSH terms
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14-3-3 Proteins / genetics*
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14-3-3 Proteins / metabolism
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3-Mercaptopropionic Acid / chemistry
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Actins / genetics
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Actins / metabolism
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Cadmium Compounds / pharmacology
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Ferric Compounds / pharmacology
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Gene Expression Regulation / drug effects*
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Humans
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Leukocytes, Mononuclear / cytology
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Leukocytes, Mononuclear / drug effects*
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Leukocytes, Mononuclear / metabolism
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Magnetite Nanoparticles / chemistry*
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Peptidylprolyl Isomerase / genetics
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Peptidylprolyl Isomerase / metabolism
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Polymerase Chain Reaction / standards*
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Primary Cell Culture
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Quantum Dots / chemistry*
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Reference Standards
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Selenium Compounds / pharmacology
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Sulfides / pharmacology
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Ubiquitin C / genetics
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Ubiquitin C / metabolism
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Zinc Compounds / pharmacology
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beta 2-Microglobulin / genetics
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beta 2-Microglobulin / metabolism
Substances
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14-3-3 Proteins
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Actins
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Cadmium Compounds
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Ferric Compounds
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Magnetite Nanoparticles
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Selenium Compounds
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Sulfides
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Ubiquitin C
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YWHAZ protein, human
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Zinc Compounds
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beta 2-Microglobulin
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ferric oxide
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cadmium selenide
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3-Mercaptopropionic Acid
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Peptidylprolyl Isomerase
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zinc sulfide