The AMSH3 ESCRT-III-Associated Deubiquitinase Is Essential for Plant Immunity

Cell Rep. 2018 Nov 27;25(9):2329-2338.e5. doi: 10.1016/j.celrep.2018.11.011.

Abstract

Plant "nucleotide-binding leucine-rich repeat" receptor proteins (NLRs) detect alterations in host targets of pathogen effectors and trigger immune responses. The Arabidopsis thaliana mutant pen1 syp122 displays autoimmunity, and a mutant screen identified the deubiquitinase "associated molecule with the SH3 domain of STAM3" (AMSH3) to be required for this phenotype. AMSH3 has previously been implicated in ESCRT-mediated vacuolar targeting. Pathology experiments show that AMSH3 activity is required for immunity mediated by the CC-NLRs, RPS2 and RPM1. Co-expressing the autoactive RPM1D505V and the catalytically inactive ESCRT-III protein SKD1E232Q in Nicotiana benthamiana supports the requirement of ESCRT-associated functions for this CC-NLR-activated immunity. Meanwhile, loss of ESCRT function in A. thaliana is lethal, and we find that AMSH3 knockout-triggered seedling lethality is "enhanced disease susceptibility 1" (EDS1) dependent. Future studies may reveal whether AMSH3 is monitored by a TIR-NLR immunity receptor.

Keywords: AMSH3 deubiquitinase; ESCRT; autoimmunity; nucleotide-binding leucine-rich repeat receptors; plant immunity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis
  • Arabidopsis / immunology*
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Endosomal Sorting Complexes Required for Transport / metabolism*
  • Lysine / metabolism
  • Phenotype
  • Plant Immunity*
  • Signal Transduction
  • Ubiquitin-Specific Proteases / metabolism*

Substances

  • Arabidopsis Proteins
  • Endosomal Sorting Complexes Required for Transport
  • Ubiquitin-Specific Proteases
  • AMSH3 protein, Arabidopsis
  • Lysine