Protocols for Isolation and Evaluation of Muse Cells

Kazuki Tatsumi; Yoshihiro Kushida; Shohei Wakao; Yasumasa Kuroda; Mari Dezawa

doi:10.1007/978-4-431-56847-6_4

Protocols for Isolation and Evaluation of Muse Cells

Adv Exp Med Biol. 2018:1103:69-101. doi: 10.1007/978-4-431-56847-6_4.

Authors

Kazuki Tatsumi^{1

2}, Yoshihiro Kushida¹, Shohei Wakao¹, Yasumasa Kuroda¹, Mari Dezawa³

Affiliations

¹ Department of Stem Cell Biology and Histology, Tohoku University Graduate School of Medicine, Sendai, Japan.
² Analytical Research Department, Technology Development Unit, Regenerative Medicine Division, Life Science Institute, Inc., Tokyo, Japan.
³ Department of Stem Cell Biology and Histology, Tohoku University Graduate School of Medicine, Sendai, Japan. mdezawa@med.tohoku.ac.jp.

PMID: 30484224
DOI: 10.1007/978-4-431-56847-6_4

Abstract

This chapter provides the detailed method for isolation of Muse cells and evaluation of their pluripotency. The basic population of Muse cells is cultured mesenchymal stem cells such as bone marrow-mesenchymal stem cells, fibroblasts, and adipose-derived stem cells. The detailed method for handling mesenchymal stem cells is also provided in this protocol.

Keywords: Adipose-derived stem cells; Anti-SSEA-3 antibody; Cell sorting; Clusters; FACS; Fibroblasts; MSCs; Serum lot check.

Publication types

Review

MeSH terms

Adipocytes / cytology
Bone Marrow Cells / cytology
Cell Separation / methods*
Cells, Cultured
Fibroblasts / cytology
Humans
Mesenchymal Stem Cells / cytology
Pluripotent Stem Cells / cytology*