Isolation of a sleep-promoting compound from Polygonatum sibiricum rhizome

Kyungae Jo; Hoon Kim; Hyeon-Son Choi; Seung-Su Lee; Myun-Ho Bang; Hyung Joo Suh

doi:10.1007/s10068-018-0431-0

Isolation of a sleep-promoting compound from Polygonatum sibiricum rhizome

Food Sci Biotechnol. 2018 Jul 13;27(6):1833-1842. doi: 10.1007/s10068-018-0431-0. eCollection 2018 Dec.

Authors

Kyungae Jo¹, Hoon Kim², Hyeon-Son Choi³, Seung-Su Lee², Myun-Ho Bang², Hyung Joo Suh¹

Affiliations

¹ 1Department of Public Health Science, Korea University, Seoul, 07249 Republic of Korea.
² 2Skin Biotechnology Center, Kyung Hee University, Yongin, Gyeonggi 16229 Republic of Korea.
³ 3Department of Food Science and Technology, Seoul Women's University, Seoul, 01797 Republic of Korea.

Abstract

The aim of this study was to identify sleep-promoting substance from Polygonatum sibiricum rhizome extract (PSE) with the regulation of sleep architecture. PSE showed a decrease in sleep latency time and an increase in the sleeping time. In the electroencephalography analysis of rats, PSE (150 mg/kg) showed an increase of non-rapid eye movement by 38% and a decrease of rapid eye movement by 31% compared to the control. This sleep-promoting activity was found to be involved in the GABA_A-BDZ receptor. The chemical structure of the pure compound was determined by the ¹H and ¹³C nuclear magnetic resonance spectroscopy and gas chromatography mass spectrometry analysis; active compound was glyceryl-1-monolinoleate. The commercial standard glyceryl-1-monolinoleate showed a similar inhibitory concentration on [³H]-flumazenil binding to GABA_A-BDZ receptors with final active fraction of PSE. The results indicate that glyceryl-1-monolinoleate is a major active compound responsible for the PSE-derived sleep promotion.

Keywords: GABA type A-benzodiazepine receptor; Glyceryl monolinoleate; Insomnia; Polygonatum sibiricum; Sleep.