Imaging of intracellular-specific microRNA in tumor cells by symmetric exponential amplification-assisted fluorescence in situ hybridization

Jun Chen; Wen Yin; Yingjun Ma; Huihui Yang; Yanfei Zhang; Mengfei Xu; Xuling Zheng; Zong Dai; Xiaoyong Zou

doi:10.1039/c8cc08849g

Imaging of intracellular-specific microRNA in tumor cells by symmetric exponential amplification-assisted fluorescence in situ hybridization

Chem Commun (Camb). 2018 Dec 11;54(99):13981-13984. doi: 10.1039/c8cc08849g.

Authors

Jun Chen¹, Wen Yin, Yingjun Ma, Huihui Yang, Yanfei Zhang, Mengfei Xu, Xuling Zheng, Zong Dai, Xiaoyong Zou

Affiliation

¹ School of Chemistry, Sun Yat-Sen University, Guangzhou 510275, China.

PMID: 30480275
DOI: 10.1039/c8cc08849g

Abstract

We report a symmetric exponential amplification-assisted fluorescence in situ hybridization (SEXPAR-FISH) strategy for imaging intracellular-specific microRNAs (miRNAs) in cells. The strategy eliminates the risk of cell loss and miRNA leakage, and low-abundant intracellular miR-27a in cells can be imaged in only 2 h without compromising sensitivity and specificity.

MeSH terms

Cell Line, Tumor
Humans
In Situ Hybridization, Fluorescence / methods*
Limit of Detection
Lung Neoplasms / genetics
Lung Neoplasms / pathology
MicroRNAs / genetics*
Molecular Imaging / methods*
Nucleic Acid Amplification Techniques / methods*
Proof of Concept Study
Templates, Genetic

Substances

MicroRNAs