Differential Expression of Sumoylation Enzymes SAE1, U BA2, UBC9, PIAS1 and RanBP2 in Major Ocular Tissues of Mouse Eye

Qian Nie; Lan Yang; Wenjie Qing; Jie Xie; Xiaodong Gong; Huimin Chen; Yuwen Gan; Ling Wang; Jia-Wen Xiang; Yuan Xiao; Zhigang Chen; David Wan-Cheng Li

doi:10.2174/1566524018666181116112208

Differential Expression of Sumoylation Enzymes SAE1, U BA2, UBC9, PIAS1 and RanBP2 in Major Ocular Tissues of Mouse Eye

Curr Mol Med. 2018;18(6):376-382. doi: 10.2174/1566524018666181116112208.

Authors

Qian Nie^{1

2}, Lan Yang^{1

2}, Wenjie Qing^{1

2}, Jie Xie^{1

2}, Xiaodong Gong², Huimin Chen^{1

2}, Yuwen Gan^{1

2}, Ling Wang², Jia-Wen Xiang^{1

2}, Yuan Xiao^{1

2}, Zhigang Chen², David Wan-Cheng Li^{1

2}

Affiliations

¹ Key Laboratory of Protein Chemistry and Developmental Biology, College of Life Sciences, Hunan Normal University, Changsha, Hunan 410081, China.
² The State Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, #54 Xianlie South Road, Guangzhou, Guangdong 510060, China.

PMID: 30479214
DOI: 10.2174/1566524018666181116112208

Abstract

Background: It is now well established that protein sumoylation is an important mechanism to regulate multiple cellular processes including gene transcription, chromatin structure, cell proliferation and differentiation, as well as pathogenesis.

Objective: In the vertebrate eye, we and others have previously shown that sumoylation can regulate differentiation of major ocular tissues including retina and lens. However, the expression patterns of the three types of sumoylation enzymes, the activating enzymes SAE1 and UBA2, the conjugating enzyme UBC9, and the ligating enzymes such as RanBP2 and PIAS1 have not been well studied in the ocular tissues.

Conclusion: In the present study, using QRT-PCR and western blot analysis, we have determined the differentiatial expression patterns of the above three types of enzymes, and the obtained results lay down a foundation for further exploration of sumoylation functions in vertebrate eye.

Keywords: PIAS1; RanBP2; SAE1; Sumoylation; UBA2; UBC9; sumoylation enzymes..

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Eye Proteins / biosynthesis*
Female
Gene Expression Regulation / physiology*
Lens, Crystalline / cytology
Lens, Crystalline / metabolism*
Male
Mice
Molecular Chaperones / biosynthesis*
Nuclear Pore Complex Proteins / biosynthesis*
Protein Inhibitors of Activated STAT / biosynthesis*
Retina / cytology
Retina / metabolism*
Sumoylation / physiology*
Ubiquitin-Activating Enzymes / biosynthesis*
Ubiquitin-Conjugating Enzymes / biosynthesis*

Substances

Eye Proteins
Molecular Chaperones
Nuclear Pore Complex Proteins
Pias1 protein, mouse
Protein Inhibitors of Activated STAT
SAE1 protein, mouse
ran-binding protein 2
Ubiquitin-Conjugating Enzymes
Ubiquitin-Activating Enzymes
ubiquitin-conjugating enzyme UBC9